Effects of Ethanol and its Metabolites on Responses of the Rat Bladder Muscle

Kim, Hyung Jee; Moon, Hyun; Lee, Gil Ho

Abstract

PURPOSE: This study was designed to investigate the direct effects of ethanol and its metabolites, acetaldehyde and acetic acid, on isolated rat bladder muscle.
MATERIALS AND METHODS
For measuring of isometric tension change, rat bladder muscle strips were used. Following ethanol(0.25-3%) was applied to bethanechol(0.1mM)-precontracted muscle strips, acetaldehyde(0.1 to 10mM) and acetic acid(0.1 to 10mM) were applied to resting muscle strips respectively, and were applied to bethanechol(0.1 mM)-precontracted muscle strips respectively.
RESULTS
Bladder contraction of tissue strips induced by bethanechol was reduced by ethanol in a dose-dependent manner. Acetaldehyde(1 to 10mM) and acetic acid(5 to 1 0mM) increased the rssting tension(control group), and acetaldehyde(0.1 to 10mM) and acetic acid(0.1 to 10mM) applied to bethanechol-induced contraction enhanced significantly contraction compared to control group.
CONCLUSIONS
The results of this study indicate that responses of the rat bladder muscle were reduced by exposure to ethanol, and however, acetaldehyde and acetic acid affect the resting tension itself and show synergistic effect to bethanechol in bladder contractility. These suggest that all of the acute ethanol intoxication did not cause to urinary retention in human with benign prostatic hyperplasia.