Korean J Physiol Pharmacol.  1997 Dec;1(6):825-830.

The effect of rebamipide on cellular release of leukotriene B4 by Helicobacter pylori

• Lee JJ
• Han BG
• Ro JY
• Rhee KH
• Youn HS
• Kim MN
• Chung MH

Abstract

Leukotrienes (LTs) are known to act as a mediator provoking tissue response in inflammation. This finding implicates that LTs also play important roles in the pathogenesis of H. pylori-induced gastritis and gastric ulceration. Rebamipide is being currently used as a therapeutics for gastritis and peptic ulcer, but their mechanisms of action have not been known clearly yet. One possibility is that their therapeutic effects are ascribed to interfering with the H. pylori-induced release of LTs from neutrophils and gastric mucosal cells. In the present study, this possibility was tested using LTB4 as the test material in human neutrophils and Kato III cells(gastric adenoma cells as a substitute for gastric mucosal cells). The release of LTB4 from both neutrophils and Kato III cells was time and H. pylori-dose dependent. The maximum release of LTB4 was induced by neutrophils and Kato III cells when these cells incubated with H. pylori 4.8 X 108 cells/ml for 30 min. But in the presence of rebamipide the release of LTB4 from these cells was suppressed in dose dependent manners. The release was completely suppressed at 1.0 mM of rebamipide in neutrophils and 2.0 mM of this drug in Kato III cells, respectively. We also obtained the results that the release of LTB4 was induced by A23187 (Ca2+ ionophore) and the A23187-induced release was also inhibited by rebamipide. It seems that the mechanism of action of rebamipide is through its interaction with the level of intracellular Ca2+. In view of the roles of LTB4 in inflammatory reaction and the roles of H. pylori in gastritis and peptic ulcer, the effects of this drug observed in this study may contribute to their therapeutic action in these gastric disorders.

Keyword

Helicobacter pylori; Leukotriene B4; Rebamipide

MeSH Terms

Adenoma
Calcimycin
Gastritis
Helicobacter pylori*
Helicobacter*
Humans
Inflammation
Leukotriene B4*
Leukotrienes
Neutrophils
Peptic Ulcer
Stomach Ulcer
Calcimycin
Leukotriene B4
Leukotrienes