Korean J Obstet Gynecol.  2001 Jun;44(6):1040-1050.

Effect of superovulation on the expression of Tissue Inhibitor of Metalloproteinases-3 and Matrix Metalloproteinases-9 in murine endometrium

• Ku SY
• Choi YM
• Kim YS
• Lee JY

Abstract

OBJECTIVES
The effects of superovulation on the expression of mRNA and protein of TIMP-3 and MMP-9 in murine endometrium were assessed.
METHODS
Using murine pregnant uteri of gestation day (g.d.) 4, 5 and 6 after injection of PMSG 5 and 10 IU, the effects of superovulation were assessed and compared with those of natural pregnancy and pseudopregnancy groups using quantitative competitive RT-PCR and immunohistochemical staining.
RESULTS
Expression of TIMP-3 mRNA and protein showed an increase in PMSG groups and pseudopregnancy group, while there was no difference in MMP-9 expression between natural pregnancy and PMSG, pseudopregnancy groups on g.d. 4 through g.d. 6.
CONCLUSIONS
This study suggests that ovarian hyperstimulation by gonadotropin, which produces many oocytes and embryos, may have a detrimental effect on embryonic implantation and its relevant endometrial remodeling process by increase in expression of TIMP-3 in murine endometrium.

Keyword

Superovulation; TIMP-3; MMP-9; Endometrium; Mouse

MeSH Terms

Animals
Embryonic Structures
Endometrium*
Female
Gonadotropins
Mice
Oocytes
Pregnancy
Pseudopregnancy
RNA, Messenger
Superovulation*
Tissue Inhibitor of Metalloproteinase-3
Uterus
Gonadotropins
RNA, Messenger
Tissue Inhibitor of Metalloproteinase-3