Korean J Nephrol.  1997 Jun;16(2):230-237.

Localization of Dopamine D1 and D2 Receptor Protein Using Immunohistochemistry in Rat Kidneys

• Chang SH
• Han JS

Abstract

Dopamine receptors in the CNS and other several tissues were identified by physiological, biochemical and radioligand binding techniques. But previous morphological and biochemical studies have been unable to charaterize or determine the tissue distribution of dopamine receptor subtypes because no selective ligands are available yet. Furthermore, the cellular distribution of the dopamine receptor subtypes in the rat kidney is not demonstrated well. The present study utilizes specific antibodies to characterize the renal distribution of this dopamine receptor subtype using light microscopic immunohistochemistry in the rat kidney. In the rat kidney, D1 receptor protein was localized to proximal tubule, distal tubule, renal vessels, medullary collecting tubule, juxtaglomerular apparatus(JGA) and glomerulus. And D2 receptor protein was localized to distal tubule, Henle's loop, proximal tubule, medullary collecting tubules, juxtaglomerular apparatus(JGA) and renal vasculature. The D1 and D2 receptors, which present in the central nervous system, are now identified in the rat kidney. There are some differences in receptors expressing sites on the previous radioligand binding and pharmacologic studies, but these results suggest that at least some of the renal dopamine DA1 and DA2 receptors correspond structually to the central dopamine D1 and D2 receptors.

Keyword

Dopamine receptor; Immunohistochemistry; Kidney; Rat

MeSH Terms

Animals
Antibodies
Central Nervous System
Dopamine*
Immunohistochemistry*
Kidney*
Ligands
Rats*
Receptors, Dopamine
Tissue Distribution
Antibodies
Dopamine
Ligands
Receptors, Dopamine