Korean J Asthma Allergy Clin Immunol.  2006 Sep;26(3):200-205.

IL-12 Production in Lipopolysaccharide- and Dermatophagoides pteronyssinus- Copulsed Monocyte-derived Dendritic Cells


BACKGROUND: Epidemiological studies have provided indirect evidence that exposure to less invasive microbials enhances the development of allergic diseases. Nonetheless, our understanding of the mechanisms that underlie this apparent symbiosis between microbes and allergen remains incomplete.
The purpose of our study was to evaluate the response of Dermatophagoides pteronyssinus (Der p) on Lipopolysaccharide (LPS) pulsed Dendritic cells (DCs). METHOD: Peripheral blood monocyte-derived DCs were obtained from 11 healthy donors. The monocyte-derived DCs were cultured with the LPS and Der p. Then, the costimulatory molecule expression and IL-12 production were evaluated. RESULT: Der p-activated monocyte-derived DCs did not induce the costimulatory molecule expression and IL-12 production. In contrast, LPS-activated monocyte-derived DCs were found to produce IL-12 in a concentration-dependent manner and to increase their expression of CD80 and CD86. However, there was no differences between 'LPS pulsed DCs' and 'LPS and Der p copulsed DCs' in the amount of IL-12 produced.
In healthy adults, LPS-induced IL-12 production was not impaired by Der p.

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