J Korean Ophthalmol Soc.  2011 Jun;52(6):671-678. 10.3341/jkos.2011.52.6.671.

Effects of Mitomycin C on Scleral Collagen Fibrils According to Atomic Force Microscopy

Affiliations
  • 1Department of Ophthalmology, School of Medicine, Kangwon National University, Chuncheon, Korea. opticus@kangwon.ac.kr
  • 2Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Seoul, Korea.
  • 3Department of Ophthalmology, College of Medicine, Kyung Hee University, Seoul, Korea.
  • 4Program of Medical Engineering, Kyung Hee University, Seoul, Korea.

Abstract

PURPOSE
To investigate the effects of mitomycin C on the scleral collagen surfaces using atomic force microscopy (AFM).
METHODS
Two non-contact mode AFM machines were used to observe changes in the morphological characteristics of human scleral surfaces before and after one, three, and five minutes of 0.02% mitomycin C application. Based on AFM topography and deflection images of the collagen fibril, the morphological characteristics of scleral fibrils including the fibril diameter and D-period were measured using the line profile.
RESULTS
The sclera collagen fibril treated with 0.02% mitomycin C for one minute did not show any significant increases in mean fibril diameter (155.04 +/- 17.46 nm) or mean D-periodicity (70.02 +/- 3.33 nm), compared to those of the control group. However, the scleral collagen fibrils treated with 0.02% mitomycin C for three and five minutes showed significant increases in mean fibril diameter (182.33 +/- 16.33 nm, 199.20 +/- 12.40 nm, respectively) and mean D-periodicity (70.27 +/- 13.66 nm, 72.75 +/- 19.32 nm, respectively), compared to those of the control group.
CONCLUSIONS
The present study examined the structural changes in the scleral collagen fibrils before and after mitomycin C application according to atomic force microscopy. The results indirectly suggest that three or more minutes of 0.02% mitomycin C application affects the morphology of scleral collagen.

Keyword

Atomic force microscopy; Collagen fibrils; Diameter; D-banding; Human sclera; Mitomycin C
Full Text Links
  • JKOS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2021 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr