J Korean Ophthalmol Soc.  1995 Apr;36(4):719-724.

Ultrastructural Changes of The Scleral Fibroblasts after Glaucoma Filtering Surgery with Intraoperative Mitomycin-C in Rabbit Eyes

  • 1Department of Ophthalmology, St. Mary's Hospital, Catholic University, Medical College, Seoul, Korea.


We investigated the long-term scleral toxicity after single intraoperative exposure to mitomycin-C(MMC) at 2 different exposure length of time and with or without profuse irrigation after the MMC applications during glaucoma filtering surgery(GFS) in rabbit eyes. Albino rabbits undergoing GFS received a single exposure to MMC 0.5 mg/ml for 1 or 5 minutes with placing MMC-soaked sponges between the conjunctival flap and the scleral bed. A subsequent irrigation of the exposed tissue with 250 ml of balanced salt solution(BSS) was done in one group but not in the other groups. Six months after GFS, the eyes were enucleated and fixed in glutaraldehyde for transmission electron microscopy(TEM). TEM revealed degenerative changes of the fibroblasts at the middle portion of sclera in all MMC treated groups. There were some differences between the groups with 1 minute and 5 minutes exposure to MMC. No discernible differences existed in the ultrastructural findings of the scleral fibroblasts between the groups with or without BSS irrgation after 5-minutes exposure to MMC. Our observation suggest that an exposure to MMC 0.5 mg/ml can cause longterm scleral fibroblast toxicity. There was no discernible differences in scleral fibroblast toxicity between the groups with and without profuse BSS irrigation.


Glaucoma filtering surgery(GFS); Mitomycin-C(MMC); Scleral toxicity
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