J Periodontal Implant Sci.  2011 Feb;41(1):10-16. 10.5051/jpis.2011.41.1.10.

Effect of (-)-epigallocatechin-3-gallate on maintaining the periodontal ligament cell viability of avulsed teeth: a preliminary study

• Jung IH
• Yun JH
• Cho AR
• Kim CS
• Chung WG
• Choi SH

• KMID: 2212130
• DOI: http://doi.org/10.5051/jpis.2011.41.1.10

Abstract

PURPOSE
Avulsed tooth can be completely recovered, if sound periodontal ligament (PDL) of tooth is maintained. Although a lot of storage solutions have been explored for the better storage of avulsed tooth, there is a shortcoming that the preservation time is much short. On the other hand, there has been studies that (-)-epigallocatechin-3-gallate (EGCG), the most abundant polyphenol in green tea, which is related to the anti inflammatory, antioxygenic, and antibacterial effects, allows the successful preservations of tissues and cells. This study evaluated the effect of EGCG on avulsed-teeth preservation of Beagle dogs for a period of time.
METHODS
The atraumatically extracted teeth of Beagle dogs were washed and preserved with 0/10/100 microM of EGCG at the time of immediate, period 1 (4 days in EGCG-contained media and additional 1 day in EGCG-free media), period 2 (8 days in EGCG-contained media and additional 2 days in EGCG-free media) and period 3 (12 days in EGCG-contained media and additional 2 days in EGCG-free media). Then, the cell viabilities of preserved teeth was calculated by dividing optical density (OD) of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay with OD of eosin assay to eliminate the measurement errors caused by the different tissue volumes.
RESULTS
From the results, the immediately analyzed group presented the highest cell viability, and the rate of living cells on teeth surface decreased dependent on the preservation period. However, the 100 microM of EGCG-treated group showed statistically significant positive cell activity than EGCG-free groups throughout preservation periods.
CONCLUSIONS
Our findings showed that 100 microM EGCG could maintain PDL cell viability of extracted tooth. These results suggest that although EGCG could not be a perfect additive for tooth preservation, it is able to postpone the period of tooth storage. However, further in-depth studies are required for more plausible use of EGCG.

Keyword

Epigallocatechin gallate; Organ preservation; Tooth replantation; MTT formazan

MeSH Terms

Animals
Catechin
Cell Survival
Dogs
Eosine Yellowish-(YS)
Formazans
Hand
Organ Preservation
Periodontal Ligament
Tea
Tetrazolium Salts
Thiazoles
Tooth
Tooth Avulsion
Tooth Replantation
Catechin
Eosine Yellowish-(YS)
Formazans
Tea
Tetrazolium Salts
Thiazoles

Figure

• 

  Figure 1 Extraction and preservation of teeth from Beagle dogs. The teeth of Beagle dogs were extracted atraumatically (A) and preserved in 24-well plates in storage media containing (-)-epigallocatechin-3-gallate at various concentrations (B).

• 

  Figure 2 Changes in cell viability of the tooth surface, according to the preservation period. The teeth were treated with 0, 10, or 100 µM (-)-epigallocatechin-3-gallate (EGCG), after which cell-viability assays (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were performed at various points in time: immediate, period 1 (preservation with EGCG for 4 days and one further day without it), period 2 (for 8 days with EGCG and 2 further days without), and period 3 (for 12 days with EGCG and 2 further days without). Data are mean±SD values. Uppercase letter: comparisons among concentrations of EGCG (P<0.05). Lowercase letter: comparisons among analysis periods (P<0.05).

• 

  Figure 3 Differences in cell viability according to the concentration of (-)-epigallocatechin-3-gallate (EGCG). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays were performed for the extracted teeth after periods 1 (A), 2 (B), and 3 (C). The teeth were treated with 0, 10, or 100 µM EGCG for 4, 8, or 12 days depending on the period. Data are mean±SD values. a) Statistically significant difference between those teeth treated with 0 and 100 µM EGCG (P<0.1). b) Statistically significant difference between those teeth treated with 0 and 100 µM EGCG (P<0.05).

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