Korean J Orthod.  2006 Dec;36(6):465-473.

Effects of nitric oxide on the proliferation and differentiation of human periodontal ligament cells

Affiliations
  • 1Department of Orthodontics, School of Dentistry, Wonkwang University, Korea. occlplane@hanmail.net

Abstract


OBJECTIVE
This study evaluated the effects of nitric oxide (NO) on the proliferation and differentiation of human periodontal ligament cells involved in orthodontic tooth movement.
METHODS
A range of concentrations of sodium nitroprusside (SNP), a NO donor, were administered to samples of human periodontal ligament cells, followed by measurement of cell viability, alkaline phosphatase (ALP) activity, and expression of osteonectin and bone sialoprotein.
RESULTS
Cell viability, ALP activity and the expression of osteonectin and bone sialoprotein were increased in human periodontal ligament cells treated with SNP concentrations of < 0.2 mM compared with controls, but were decreased with SNP concentrations of > 1.0 mM.
CONCLUSION
NO has a biphasic effect on proliferation and differentiation in human periodontal ligament cells, with a stimulatory effect at low concentrations and an inhibitory effect at high concentrations.

Keyword

Nitric oxide; Human periodontal ligament cells; Growth and differentiation

MeSH Terms

Alkaline Phosphatase
Cell Survival
Humans*
Integrin-Binding Sialoprotein
Nitric Oxide*
Nitroprusside
Osteonectin
Periodontal Ligament*
Tissue Donors
Tooth Movement
Alkaline Phosphatase
Integrin-Binding Sialoprotein
Nitric Oxide
Nitroprusside
Osteonectin
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