J Korean Acad Conserv Dent.  2007 Mar;32(2):95-101. 10.5395/JKACD.2007.32.2.095.

Pulp response of mineral trioxide aggregate, calcium sulfate or calcium hydroxide

• Yun YR
• Yang IS
• Hwang YC
• Hwang IN
• Choi HR
• Yoon SJ
• Kim SH
• Oh WM

• KMID: 2175842
• DOI: http://doi.org/10.5395/JKACD.2007.32.2.095

Abstract

This study was performed to verify the possibility of MTA and calcium sulfate as a pulp capping agent through comparing the dental pulp response in dogs after capping with MTA, calcium sulfate, and calcium hydroxide. 24 teeth of 2 dogs, 8 month old, were used in this study. Under general anesthesia, cervical cavities were prepared and pulp was exposed with sterilized #2 round bur in a high speed handpiece. MTA, calcium hydroxide, and calcium sulfate were applied on the exposed pulp. Then the coronal openings were sealed with IRM and light-cured composite. Two months after treatment, the animals were sacrificed. The extracted teeth were fixed in 10% neutral-buffered formalin solution and were decalcified in formic acid-sodium citrate. They were prepared for histological examination in the usual manner. The sections were stained with haematoxylin and eosin. In MTA group, a hard tissue bridges formation and newly formed odontoblasts layer was observed. There was no sign of pulp inflammatory reaction in pulp tissue. In calcium hydroxide group, there was no odontoblast layer below the dentin bridge. In pulpal tissue, chronic inflammatory reaction with variable intensity and extension occurred in all samples. In calcium sulfate group, newly formed odontoblast layer was observed below the bridge. Mild chronic inflammation with a few neutrophil infiltrations was observed on pulp tissue. These results suggest that MTA is more biocompatible on pulp tissue than calcium hydroxide or calcium sulfate.

Keyword

MTA; Calcium sulfate; Calcium hydroxide; Pulp capping

MeSH Terms

Anesthesia, General
Animals
Calcium Hydroxide*
Calcium Sulfate*
Calcium*
Citric Acid
Dental Pulp
Dental Pulp Capping
Dentin
Dogs
Eosine Yellowish-(YS)
Formaldehyde
Humans
Infant
Inflammation
Neutrophils
Odontoblasts
Pulp Capping and Pulpectomy Agents
Tooth
Pemetrexed
Calcium
Calcium Hydroxide
Calcium Sulfate
Citric Acid
Eosine Yellowish-(YS)
Formaldehyde
Pulp Capping and Pulpectomy Agents

Figure

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  Figure 1 Histological section of maxillary canine of dog after pulp capping with MTA (Haematoxylin and eosin stain: original magnification × 40). Black arrow indicates newly formed hard tissue. Black arrow head indicates MTA particle.

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  Figure 2 Histological section of mandibular canine of dog after pulp capping with MTA (Haematoxylin and eosin stain: original magnification × 200). Between dentin bridges and pulp tissues, newly odontoblastic layer were well developed.

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  Figure 3 Histological section of mandibular molar of dog after pulp capping with Calcium hydroxide (Haematoxylin and eosin stain: original magnification × 100).

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  Figure 4 Histological section of mandibular molar of dog after pulp capping with CaSO4 (Haematoxylin and eosin stain: original magnification × 200).

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