Blood Res.  2014 Mar;49(1):49-53. 10.5045/br.2014.49.1.49.

The caspase-3 inhibitor (peptide Z-DEVD-FMK) affects the survival and function of platelets in platelet concentrate during storage

• Shiri R
• Yari F
• Ahmadinejad M
• Vaeli S
• Tabatabaei MR

• KMID: 2172823
• DOI: http://doi.org/10.5045/br.2014.49.1.49

Abstract

BACKGROUND
Although apoptosis occurs in nucleated cells, studies show that this event also occurs in some anucleated cells such as platelets. During storage of platelets, the viability of platelets decreased, storage lesions were observed, and cells underwent apoptosis. We investigated the effects of caspase-3 inhibitor on the survival and function of platelets after different periods of storage.
METHODS
Platelet concentrates were obtained from the Iranian Blood Transfusion Organization in plastic blood bags. Caspase-3 inhibitor (Z-DEVD-FMK) was added to the bags. These bags along with control bags to which no inhibitor was added were stored in a shaking incubator at 22degrees C for 7 days. The effects of Z-DEVD-FMK on the functionality of platelets were analyzed by assessing their ability to bind to von Willebrand factor (vWF) and to aggregate in the presence of arachidonic acid and ristocetin. Cell survival was surveyed by MTT assay.
RESULTS
At day 4 of storage, ristocetin-induced platelet aggregation was significantly higher in the inhibitor-treated (test) than in control samples; the difference was not significant at day 7. There was no significant difference in arachidonic acid-induced platelet aggregation between test and control samples. However, at day 7 of storage, the binding of platelets to vWF was significantly higher in test than in control samples. The MTT assay revealed significantly higher viability in test than in control samples at both days of study.
CONCLUSION
Treatment of platelets with caspase-3 inhibitor could increase their functionality and survival.

Keyword

Platelet concentrate; Storage; Caspase inhibitor; von Willebrand factor

MeSH Terms

Apoptosis
Arachidonic Acid
Blood Platelets*
Blood Transfusion
Caspase 3*
Cell Survival
Incubators
Plastics
Platelet Aggregation
Ristocetin
von Willebrand Factor
Arachidonic Acid
Caspase 3
Plastics
Ristocetin
von Willebrand Factor

Figure

• 

  Fig. 1 Flow cytometry plot. The vWF binding capacity of platelets at the days 4 and 7 of storage. (A) The platelets were gated, (B) the binding levels in the negative control in the absence of antibodies to vWF, (C) the vWF binding levels of platelets in the presence of caspase-3 inhibitor at the day 4 of storage, (D) the vWF binding levels of platelets without caspase-3 inhibitor at the day 4 of storage, and (E) the vWF binding levels of platelets in the presence of caspase-3 inhibitor at the day 7 of storage, (F) the vWF binding levels of platelets without caspase-3 inhibitor at the day 7 of storage.

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