Blood Res.  2014 Sep;49(3):177-181. 10.5045/br.2014.49.3.177.

Total extract of Korean red ginseng facilitates human bone marrow hematopoietic colony formation in vitro

Affiliations
  • 1Department of Laboratory Medicine, Catholic University of Daegu, School of Medicine, Daegu, Korea. ajlee388@gmail.com
  • 2Division of Hematology/Oncology, Department of Internal Medicine, Catholic University of Daegu, School of Medicine, Daegu, Korea.
  • 3Department of Oriental Internal Medicine of Hepatology, College of Oriental Medicine and Daegu Hanny University, Daegu, Korea.

Abstract

BACKGROUND
The number of CD34+ cells in a peripheral blood stem cell collection is the key factor in predicting successful treatment of hematologic malignancies. Korean Red Ginseng (KRG) (Panax ginseng C.A. Meyer) is the most popular medicinal herb in Korea. The objective of this study was to determine the effect of KRG on hematopoietic colony formation.
METHODS
Bone marrow (BM) samples were obtained from 8 human donors after acquiring informed consent. BM mononuclear cells (MNCs) were isolated, and CD34+ cells were sorted using magnetic beads. The sorted CD34+ cells were incubated with or without total extract of KRG (50 microg/mL, 100 microg/mL) or Ginsenoside Rg1 (100 microg/mL), and the hematopoietic colony assay was performed using methylcellulose semisolid medium. The CD34+ cell counts were measured by a single platform assay using flow cytometry.
RESULTS
The numbers of human BM-MNCs and CD34+ cells obtained after purification were variable among donors (5.6x10(7) and 1.3-48x10(7) and 8.9x10(4) and 1.8-80x10(4), respectively). The cells expanded 1,944 times after incubation for 12 d. Total extract of KRG added to the hematopoietic stem cell (HSC)-specific medium increased CD34+ cell counts 3.6 times compared to 2.6 times when using HSC medium alone. Total numbers of hematopoietic colonies in KRG medium were more than those observed in conventional medium, especially that of erythroid colonies such as burst forming unit-erythroid.
CONCLUSION
Total extract of KRG facilitated CD34+ cell expansion and hematopoietic colony formation, especially of the erythroid lineage.

Keyword

Korea traditional medicine; Ginseng; Hematopoietic colony-forming unit; CD34 antigen

MeSH Terms

Antigens, CD34
Bone Marrow*
Cell Count
Flow Cytometry
Hematologic Neoplasms
Hematopoietic Stem Cells
Humans
Informed Consent
Korea
Medicine, Korean Traditional
Methylcellulose
Panax*
Plants, Medicinal
Stem Cells
Tissue Donors
Antigens, CD34
Methylcellulose

Figure

  • Fig. 1 Total extract of Korean red ginseng added to hematopoietic stem cell-specific medium facilitated CD34+ cell expansion. Abbreviations: KRG, Korean Red Ginseng; HSC, hematopoietic stem cell.

  • Fig. 2 CFU-E colony formation in the presence of Korean red ginseng (A) and in the absence of Korean red ginseng (B).

  • Fig. 3 Korean red ginseng extract facilitated hematopoietic colony forming ability.


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