Ann Dermatol.  2011 May;23(2):177-184. 10.5021/ad.2011.23.2.177.

Epidemiologic Study of Malassezia Yeasts in Patients with Malassezia Folliculitis by 26S rDNA PCR-RFLP Analysis

• Ko JH
• Lee YW
• Choe YB
• Ahn KJ

• KMID: 2156659
• DOI: http://doi.org/10.5021/ad.2011.23.2.177

Abstract

BACKGROUND
So far, studies on the inter-relationship between Malassezia and Malassezia folliculitis have been rather scarce.
OBJECTIVE
We sought to analyze the differences in body sites, gender and age groups, and to determine whether there is a relationship between certain types of Malassezia species and Malassezia folliculitis.
METHODS
Specimens were taken from the forehead, cheek and chest of 60 patients with Malassezia folliculitis and from the normal skin of 60 age- and gender-matched healthy controls by 26S rDNA PCR-RFLP.
RESULTS
M. restricta was dominant in the patients with Malassezia folliculitis (20.6%), while M. globosa was the most common species (26.7%) in the controls. The rate of identification was the highest in the teens for the patient group, whereas it was the highest in the thirties for the control group. M. globosa was the most predominant species on the chest with 13 cases (21.7%), and M. restricta was the most commonly identified species, with 17 (28.3%) and 12 (20%) cases on the forehead and cheek, respectively, for the patient group.
CONCLUSION
Statistically significant differences were observed between the patient and control groups for the people in their teens and twenties, and in terms of the body site, on the forehead only.

Keyword

26S rDNA PCR-RFLP; Malassezia folliculitis; Malassezia yeasts

MeSH Terms

Adolescent
Cheek
DNA, Ribosomal
Epidemiologic Studies
Folliculitis
Forehead
Humans
Malassezia
Skin
Thorax
Yeasts
DNA, Ribosomal

Figure

• 

  Fig. 1 PCR-RFLP patterns of the 26S rDNA PCR, as digested with Hha I (A) and BstF51 (B), of the 11 Malassezia standard strains. Lanes: M: molecular marker, 1: M. furfur (KCTC 7743), 2: M. sympodialis (KCTC 7985), 3: M. globosa (CBS 7966), 4: M. restricta (KCTC 7848), 5: M. slooffiae (KCTC 17431), 6: M. pachydermatis (KCTC 17008), 7: M. japonica (CBS 9432), 8: M. nana (JCM 12085), 9: M. dermatis (JCM 11348), 10: M. obtusa (KCTC 7847), 11: M. yamatoensis (CBS 9725).

• 

  Fig. 2 The identified Malassezia species from the Malassezia folliculitis group and as compared with those from the control group by age.

• 

  Fig. 3 The identified Malassezia species from the Malassezia folliculitis group as compared with those from the healthy control group by the body site.

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