Korean J Anesthesiol.
2016 Feb;69(1):57-62. 10.4097/kjae.2016.69.1.57.
Lipofundin(R) MCT/LCT 20% increase left ventricular systolic pressure in an ex vivo rat heart model via increase of intracellular calcium level
- Affiliations
-
- 1Department of Anesthesiology and Pain Medicine, Gyeongsang National University Hospital, Jinju, Korea. ilwooshin@gnu.ac.kr
- 2Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Korea.
- 3Department of Anatomy and Cell biology, Dong-A University College of Medicine and Mitochondria Hub Regulation Center, Busan, Korea.
- 4Department of Anesthesiology and Pain Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
- KMID: 2152861
- DOI: http://doi.org/10.4097/kjae.2016.69.1.57
Abstract
- BACKGROUND
Lipid emulsions have been used to treat various drug toxicities and for total parenteral nutrition therapy. Their usefulness has also been confirmed in patients with local anesthetic-induced cardiac toxicity. The purpose of this study was to measure the hemodynamic and composition effects of lipid emulsions and to elucidate the mechanism associated with changes in intracellular calcium levels in myocardiocytes.
METHODS
We measured hemodynamic effects using a digital analysis system after Intralipid(R) and Lipofundin(R) MCT/LCT were infused into hearts hanging in a Langendorff perfusion system. We measured the effects of the lipid emulsions on intracellular calcium levels in H9c2 cells by confocal microscopy.
RESULTS
Infusion of Lipofundin(R) MCT/LCT 20% (1 ml/kg) resulted in a significant increase in left ventricular systolic pressure compared to that after infusing modified Krebs-Henseleit solution (1 ml/kg) (P = 0.003, 95% confidence interval [CI], 2.4-12.5). Lipofundin(R) MCT/LCT 20% had a more positive inotropic effect than that of Intralipid(R) 20% (P = 0.009, 95% CI, 1.4-11.6). Both lipid emulsion treatments increased intracellular calcium levels. Lipofundin(R) MCT/LCT (0.01%) increased intracellular calcium level more than that of 0.01% Intralipid(R) (P < 0.05, 95% CI, 0.0-1.9).
CONCLUSIONS
These two lipid emulsions had different inotropic effects depending on their triglyceride component. The inotropic effect of lipid emulsions could be related with intracellular calcium level.
Keyword
MeSH Terms
Cited by 1 articles
-
Lipid emulsion inhibits vasodilation induced by a toxic dose of bupivacaine by suppressing bupivacaine-induced PKC and CPI-17 dephosphorylation but has no effect on vasodilation induced by a toxic dose of mepivacaine
Hyunhoo Cho, Seong Ho Ok, Seong Chun Kwon, Soo Hee Lee, Jiseok Baik, Sebin Kang, Jiah Oh, Ju-Tae Sohn
Korean J Pain. 2016;29(4):229-238. doi: 10.3344/kjp.2016.29.4.229.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Lipid emulsion-mediated reversal of toxic-dose aminoamide local anesthetic-induced vasodilation in isolated rat aorta
- Anti-ischemic Effect of Polygala Tenuifolia in Isolated Rat Heart
- Role of intracellular taurine in monensin-induced Na+, Ca++ accumulation and mechanical dysfunction in isolated rat hearts
- Pain Intensity at Injection Site during Esophagogastroduodenoscopy Using Long- and MediumChain versus Long-Chain Triglyceride Propofol: A Randomized Controlled Double-Blind Study
- Cardiac response to head-out water immersion in man
