The Changes of ERG b-wave and Oscillatory Potentials in White Rats according to Dark Adaptation and Stimulus Light Intensity

Cho, Kyung Shin; Kim, Si Yeol

Abstract

Authors studied changes of ERG b-wave and oscillatory potentials(OPs) in white rats according to dark adaptation and stimulus light intensity for experimental setting. For this purpose we performed ERG tests on 10 normal white rats(20 eyes) during progressive dark adaptation. ERG b-wave and OPs values increased significantly during the first 30 minutes and then made plateau pattern. Based on this result we concluded that 30 minutes dark adaptation was sufficient for getting stable rat ERGs. After 30 minutes of dark adaptation, ERG b-wave and OPs were measured in response to 0.3J(2.25 cd.s/m2), 0.6J(6.5 cd.s/m2) , 2J(27.5 cd.s/m2) light intensity. In ERG b-wave there was no significant differene between 0.3J and 2J. In OPs between 0.3J and 0.6J, the sum of amplitude was significantly increased(p<0.01), and between 0.6J and 2J this value was insignificantly increased(p>0.05). We concluded that 0.3J(2.25 cd.s/m2) was sufficient in ERG testing. And in OPs at least 0.6J(6.5 cd.s/m2) was needed for proper stimulation.