Exp Mol Med.  1997 Mar;29(1):31-34.

Purification of thrombospondin receptor (CD36) from human platelet membrane

• Kim JH
• Suk KH
• Park NY
• Kim SH

Abstract

Thrombospondin receptor (CD36) has been recently identified in platelets and various cell lines as the receptor for thrombospondin, an adhesive protein required for irreversible aggregation of platelets as well as other adhesive processes. Thrombospondin receptor, one of major glycosylated platelet membrane proteins, is thought to play an important role as a cell adhesion molecule in blood coagulation system as well as intercellular signaling. In this work, thrombospondin receptor was purified to homogeneity from human platelet by wheat germ agglutinin (WGA)-affinity chromatography and size exclusion chromatography on Ultrogel-AcA44. The molecular weight of the purified thrombospondin receptor was about 88 kDa on SDS-PAGE and its identity was confirmed by immunoblot analysis and immunodiffusion assay.

Keyword

CD36; glycoprotein IV (GPIV); platelets; thrombosis; thrombospondin receptor

MeSH Terms

Adhesives
Antigens, CD36*
Blood Coagulation
Blood Platelets*
Cell Adhesion
Cell Line
Chromatography
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Humans*
Immunodiffusion
Membrane Proteins
Membranes*
Molecular Weight
Thrombosis
Thrombospondins*
Triticum
Adhesives
Antigens, CD36
Membrane Proteins
Thrombospondins