J Korean Soc Virol.
1999 Jun;29(2):75-86.
Serotype and Nucleotide Analysis of Human Rotavirus Isolates in Korea
- Affiliations
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- 1School of Animal Life Science, College of Animal Husbandry, Konkuk University.
- 2Jung-Bu University.
- 3Korea Research Institute of Bioscience and Biotechnology.
- 4School of Veterinary Medicine, College of Animal Husbandry, Konkuk University.
- 5Department of Microbiology, School of Medicine, Catholic University of Taegu-Hyosung.
- 6Fujita Health University, Japan.
Abstract
- Rotaviruses belong to Reoviridae causes diarrhea in human beings as well as domestic animals. This study was conducted to see what type of human rotaviruses are distributed in Seoul and Kyung-gi province. Twenty two of 81 patients showed rotavirus positive with diagnostic kit and RNA electropherosis. We isolated all of rotaviruses from the patients. Electropherotypes of 22 isolates showed 4:2:3 :2 patters whereas those migration patterns were long type. All of those isolates belonged to group 4. Twenty out of 22 isolates reacted with monoclonal antibodies specific to G1, P1A and subgroup II, whereas rest of them, 4-29 and K-30 reacted with subgroup I specific monoclonal antibody. The nucleotide sequence of an isolate K-21 showed 98~100% and 90~96% homologies with those of Wa and KU strain, respectively.
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