Korean J Obstet Gynecol.
2001 Apr;44(4):755-762.
The Total Peroxyl Radical-Trapping Ability of Amniotic Fluid in Pregnant Women with Preterm Premature Rupture of Membranes
- Affiliations
-
- 1Departments of Obstetrics and Gynecology, Chonnam National University Medical School, Kwangju, Korea.
- 2Departments of Biochemistry, Chonnam National University Medical School, Kwangju, Korea.
Abstract
OBJECTIVE
This study was to determine the levels of cytokines, lipid peroxides, and total peroxyl radical-trapping antioxidative prameter (TRAP) values of amniotic fluid in pregnant women with preterm premature rupture of membranes (PPROM). METHOD: Amniotic fluid samples were obtained by amniocenteses for chromosomal analyses and other indications(control, n=26). Amniotic fluid samples were also obtained by amnioceteses from pregnant women with preterm premature rupture of membranes (n=22). Amniotic fluid cytokines(IL-6, IL-8) were determined by enzyme-linked immunosorbent assay (ELISA). Lipid peroxide levels of amniotic fluid were measured by thiobarbituric acid (TBA) reaction. The TRAP value of amniotic fluid was assessed by measuring the rate of oxygen consuming during controlled lipid peroxidation by 2,2'-azobis-2-amidinopropane hydrochloride, a water soluble peroxyl radical generator. Ascorbic acid and uric acid were measured by high performance liquid chromatography (HPLC) CoulArray detector of water-soluble antioxidants. Retinol, alpha-tocopherol, and gamma- tocopherol were measured by HPLC-CoulArray detector of fat-soluble vitamins.
RESULTS
Cytokine levels of amniotic fluid in pregnant women with PPROM(IL-6: 23.37+/-10.61 ng/ml, IL-8: 10.23+/-3.04 ng/ml) were significantly higher than those in normal pregnant women(IL-6: 0.62+/-0.32 ng/ml, IL-8: 0.55 +/- 0.15 ng/ml), (p<0.05). Lipid peroxide levels of the amniotic fluid in pregnant women with PPROM(10.42+/-1.11 nmol/mg protein) were significantly higher than those in normal pregnant women (7.32+/-0.65 nmol/mg protein), (p<0.05). The amniotic fluid obtained from pregnant women with PPROM showed significantly lower TRAP values (0.46+/-0.04 mM) than those from normal pregnant women(0.62+/- 0.03 mM), (p<0.01). Amniotic fluid ascorbic acid levels in pregnant women with PPROM (52.1+/-15.1 nmol/ml) were significantly lower than in normal pregnant women(231.9+/-33.9 nmol/ml), (p<0.01). There were no significant differences of amniotic fluid uric acid, retinol, alpha-tocopherol, and gamma-tocopherol levels between these groups.
CONCLUSIONS
This findings suggest that a low antioxidant activity in amniotic fluid may cause PPROM and demonstrate that antioxidant vitamin, ascorbic acid, may act an important factor in the prevention of PPROM.
MeSH Terms
-
alpha-Tocopherol
Amniocentesis
Amniotic Fluid*
Antioxidants
Ascorbic Acid
Chromatography, Liquid
Cytokines
Enzyme-Linked Immunosorbent Assay
Female
gamma-Tocopherol
Humans
Interleukin-8
Lipid Peroxidation
Lipid Peroxides
Membranes*
Oxygen
Pregnant Women*
Rupture*
Tocopherols
Uric Acid
Vitamin A
Vitamins
Antioxidants
Ascorbic Acid
Cytokines
Interleukin-8
Lipid Peroxides
Oxygen
Tocopherols
Uric Acid
Vitamin A
Vitamins
alpha-Tocopherol
gamma-Tocopherol
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