Korean J Otorhinolaryngol-Head Neck Surg.  2008 May;51(5):435-440.

Inhibitory Effect of 15-Deoxy-Delta(12,14)-Prostaglandin J(2) on the MUC8 Expression in the Human Airway Epithelial Cells

Affiliations
  • 1Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, Korea. ydkim@med.yu.ac.kr

Abstract

BACKGROUND AND OBJECTIVES: Among the numerous mucin genes, MUC8 is regarded as one of the most important mucin genes which are related with upper airway disease such as a nasal polyp. 15-Deoxy-delta(12,14)-prostaglandin J(2)(15d-PGJ(2)), the most recently discovered prostaglandin, has been known to have multiple cellular functions, including anti-inflammatory and cytoprotective effects. However, the effect of 15d-PGJ(2) on mucin gene expression or mucin production has not yet been investigated. The purpose of this study was to determine the effect of 15d-PGJ(2) on interleukin-1beta (IL-1beta)-induced MUC8 gene expression and mucin secretion in the cultured NCI-H292 cells and human nasal polyp epithelial cells.
SUBJECTS AND METHOD
The MUC8 mRNA levels were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and MUC8 mucin levels were measured using an enzyme-linked immunosorbent assay (ELISA) in the cultured epithelial cells stimulated by IL-1beta. 15d-PGJ(2) was added 1 hour before stimulation.
RESULTS
15d-PGJ(2) attenuated the IL-1beta-induced MUC8 mRNA expression and mucin secretion with a dose-dependent pattern in both cultured NCI-H292 cells and human nasal polyp epithelial cells.
CONCLUSION
These results suggest that 15d-PGJ(2) may be considered as an effective agent for the control of airway mucus hypersecretion through the down-regulation of MUC8 gene.

Keyword

Nasal polyp; MUC8; Mucin; 15d-PGJ(2)

MeSH Terms

Down-Regulation
Enzyme-Linked Immunosorbent Assay
Epithelial Cells
Gene Expression
Humans
Interleukin-1beta
Mucins
Mucus
Nasal Polyps
RNA, Messenger
Interleukin-1beta
Mucins
RNA, Messenger
Full Text Links
  • KJORL-HN
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr