Korean J Urol.  2007 Sep;48(9):976-983. 10.4111/kju.2007.48.9.976.

The Expressions of p53, gamma-H(2)AX and Ku70/Ku80 That was Caused by Hydronephrosis in the Kidney of Rats

• Moon C
• Moon HY
• Kim CS

• KMID: 2061301
• DOI: http://doi.org/10.4111/kju.2007.48.9.976

Abstract

PURPOSE
There are little research about the factors of DNA damage and repair that might cause renal parenchymal damage under the condition of hydronephrosis. In this study, we studied the expressions of p53, gamma-H(2)AX and Ku70/Ku80 in rat kidney under the condition of hydronephrosis.
MATERIALS AND METHODS
16 Sprague-Dawley rats that were 7~8 weeks old were used. Partial ureteral obstruction was induced in 12 rats. And for the other 4 rats, a sham-operation was done as a control. The hydronephrosis-induced rats had their right kidney removed after 1, 2 and 3 weeks and the sham-operated rats underwent nephrectomy after 3 weeks. Those removed tissues were examined with immunohistochemical staining and western blot to confirm the degree of expression of p53, gamma-H(2)AX and Ku70/Ku80.
RESULTS
The expressions of p53, gamma-H(2)AX and Ku70/Ku80 caused by hydronephrosis in the rat increased as time passed, and these expressions of controls were in a low level or they were negative. These results were similar with the results of the immunohistochemical staining and Western blot.
CONCLUSIONS
This study confirmed that the protein expressions of gamma- H(2)AX, p53, and Ku70/Ku80 are increased, and these expressions are the DNA damage-related factors in the renal parenchyma of hydronephrosis-induced rats. We confirmed the possibility that DNA double strand breaks (DSBs) might be the main mechanism that induces renal parenchymal damage under the condition of hydronephrosis.

Keyword

Hydronephrosis; DNA damage; Double-strand DNA breaks; DNA repair

MeSH Terms

Animals
Blotting, Western
DNA
DNA Breaks, Double-Stranded
DNA Damage
DNA Repair
Hydronephrosis*
Kidney*
Nephrectomy
Rats*
Rats, Sprague-Dawley
Ureteral Obstruction
DNA

Figure

• 

  Fig. 1 Immunohistochemical staining for p53 in hydronephrosis-induced rats. The expressions of p53 after 1, 2 and 3 weeks (B, C and D) were increased compared to the control group (A), and these expressions increased with time. The expression of the tubules was higher than that of the glomerulus, and tubule expression increased with time. (A) Right kidney of the sham-operated rats after 3 weeks (control group), (B) right kidney of the hydronephrosis-induced rats after 1 week, (C) right kidney of the hydronephrosis-induced rats after 2 weeks, (D) right kidney of the hydronephrosis-induced rats after 3 weeks.

• 

  Fig. 2 Immunohistochemical staining for γ-H2AX in the hydronephrosis-induced rats. The expressions of γ-H2AX after 1, 2 and 3 weeks (B, C and D) were increased compared to the control group (A), and these expressions increased as time passed. The expression of γ-H2AX in the tubule was higher than that of glomerulus. (A) Right kidney of the shamoperated rats after 3 weeks (control group), (B) right kidney of the hydronephrosis-induced rats after 1 week, (C) right kidney of the hydronephrosis-induced rats after 2 weeks, (D) right kidney of the hydronephrosis-induced rats after 3 weeks.

• 

  Fig. 3 Immunohistochemical staining for Ku70 in hydronephrosis-induced rats. The expressions of Ku70 after 1, 2 and 3 weeks (B, C and D) were increased compared to the control group (A), and these expressions increased as time passed. The expression of Ku70 in the tubule was higher than that of the glomerulus. (A) Right kidney of the sham-operated rats after 3 weeks (control group), (B) right kidney of the hydronephrosis-induced rats after 1 week, (C) right kidney of the hydronephrosis-induced rats after 2 weeks, (D) right kidney of the hydronephrosis-induced rats after 3 weeks.

• 

  Fig. 4 Immunohistochemical staining for Ku80 in hydronephrosis-induced rats. The expressions of Ku80 after 1, 2 and 3 weeks (B, C and D) were increased compared to the control group (A), and these expressions increased as time passed. The expression of Ku80 in the tubule was higher than that of glomerulus. (A) Right kidney of the sham-operated rats after 3 weeks (control group), (B) right kidney of the hydronephrosis-induced rats after 1 week, (C) right kidney of the hydronephrosis-induced rats after 2 weeks, (D) right kidney of the hydronephrosis-induced rats after 3 weeks.

• 

  Fig. 5 Western blotting of the p53, γ-H2AX and Ku70/Ku80 expressions in the renal parenchyme of hydronephrosis-induced rats. The expressions of p53, γ-H2AX and Ku70/Ku80 were increased as time passed, and expressions of the controls were at a low level or they were negative.

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