J Korean Acad Periodontol.  1997 Dec;27(4):883-908. 10.5051/jkape.1997.27.4.883.

An Experimental Study on the Biological Specificity of Nitric Oxide and Nitric Oxide Synthetase in Periodontium-Related Cells

• Yoon HJ
• Yoon DW
• You HK
• Shin HS

• KMID: 2057014
• DOI: http://doi.org/10.5051/jkape.1997.27.4.883

Abstract

Bone remodeling is characterized by the coupling of osteoclast-mediated bone resorption and osteoblast-mediated bone formation. The process is tightly regualted at the local level by an incompletely known netwotk of peptide and non-peptide fators. Nitric oxide(NO), synthesized by nitric oxide synthetase(NOS) from L-arginine, is becoming recognized as an important bioregualtory molecule in a variety of tissue, but little is known about its possible role in periodontal tissue. The purpose of this study is to investigate the expression of nitric oxide synthetase(NOS) in inflamed gingiva and the effects of cytokine on the expression of NOS protein. The expression of NOS in gingival tissue was evaluated by immunohistochemical staining for NOS1, NOS2, NOS3. The effect of cytokine on the expression of NOS in human periodontal ligament cells and osteoblast-like HOS cells by western blot analysis. Further, we studied that NO functions in periodontal ligment cells as a regulatory molecule. PDL cells incubated with NOS inhibitor and donor. The protein expression, type I collagen & non-collagenous protein, nitrate production and cell proliferation were evaluated The results were as follows. 1. NOS1, NOS2, NOS3 was rarely distributed in healthy gingiva, but stronger stained in gingival epithelium, endothelial cells, and mononuclear cells of inflammed gingiva. 2. The cytokine stimulated NOS1, and NOS3 protein were not inducing or inhibitory effect to compared with control in PDL and HOS cells. 3.Incubation of cells with combination of TNF-alpha, IFN-gamma, LPS result in a time dependant increase in NOS2 expression, reaching a maximal level after 24 hours of stimulation. 4. The osteonectin protein inhibitory effect of NMA, inhibitor of NOS, was reversed by Larginine in dose dependant manner. 5. NMA decreased cell proliferation and nitrate production, but the inhibitory efffect of NMA was also prevented by the NO donor, sodium nitropruiside. These results suggest that exogenously synthesized NO was playing a stimulating effect on cell proliferation or on non-collagenous protein expression. Therefore NO have an important role in mediation of localized bone destruction associated inflammatory bone disease such as periodontitis

MeSH Terms

Arginine
Blotting, Western
Bone Diseases
Bone Remodeling
Bone Resorption
Cell Proliferation
Collagen Type I
Endothelial Cells
Epithelium
Gingiva
Humans
Negotiating
Nitric Oxide Synthase*
Nitric Oxide*
Osteogenesis
Osteonectin
Periodontal Ligament
Periodontitis
Sensitivity and Specificity*
Sodium
Tissue Donors
Tumor Necrosis Factor-alpha
Arginine
Collagen Type I
Nitric Oxide
Nitric Oxide Synthase
Osteonectin
Sodium
Tumor Necrosis Factor-alpha