Anat Cell Biol.  2013 Dec;46(4):254-261. 10.5115/acb.2013.46.4.254.

Ascorbic acid insufficiency induces the severe defect on bone formation via the down-regulation of osteocalcin production

Affiliations
  • 1Labratory of Vitamin C and Immunology, Department of Anatomy, Seoul National University College of Medicine, Seoul, Korea. kinglee@snu.ac.kr genius29@snu.ac.kr
  • 2Department of Rehabilitation Medicine, Seoul National University College of Medicine, Seoul, Korea.
  • 3Department of Psychology, Boston College, Chestnut Hill, MA, USA.
  • 4Division of Hematology-Oncology, Department of Internal Medicine, Ewha Womans University School of Medicine, Seoul, Korea.

Abstract

The L-gulono-gamma-lactone oxidase gene (Gulo) encodes an essential enzyme in the synthesis of ascorbic acid from glucose. On the basis of previous findings of bone abnormalities in Gulo-/- mice under conditions of ascorbic acid insufficiency, we investigated the effect of ascorbic acid insufficiency on factors related to bone metabolism in Gulo-/- mice. Four groups of mice were raised for 4 weeks under differing conditions of ascorbic acid insufficiency, namely, wild type; ascorbic acid-sufficient Gulo-/- mice, 3-week ascorbic acid-insufficient Gulo-/- mice, and 4-week ascorbic acid-insufficient Gulo-/- mice. Four weeks of ascorbic acid insufficiency resulted in significant weight loss in Gulo-/- mice. Interestingly, average plasma osteocalcin levels were significantly decreased in Gulo-/- mice after 3 weeks of ascorbic acid insufficiency. In addition, the tibia weight in ascorbic acid-sufficient Gulo-/- mice was significantly higher than that in the other three groups. Moreover, significant decreases in trabecular bone volume near to the growth plate, as well as in trabecular bone attachment to the growth plate, were evident in 3- or 4-week ascorbic acid-insufficient Gulo-/-. In summary, ascorbic acid insufficiency in Gulo-/- mice results in severe defects in normal bone formation, which are closely related to a decrease in plasma osteocalcin levels.

Keyword

L-Gulonolactone oxidase; Ascorbic acid; Osteogenesis; Osteocalcin

MeSH Terms

Animals
Ascorbic Acid*
Down-Regulation*
Glucose
Growth Plate
L-Gulonolactone Oxidase
Metabolism
Mice
Osteocalcin*
Osteogenesis*
Plasma
Tibia
Weight Loss
Ascorbic Acid
Glucose
L-Gulonolactone Oxidase
Osteocalcin
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