J Korean Acad Periodontol.  1997 Sep;27(3):459-468. 10.5051/jkape.1997.27.3.459.

Effects of some herbal drugs on gingival fibroblast and periodontal ligament cellular activity

• Doo JS
• Kang JK
• You HK
• Shin HS

• KMID: 2045117
• DOI: http://doi.org/10.5051/jkape.1997.27.3.459

Abstract

Healing of periodontal tissues require the migration and proliferation of gingival fibroblasts and periodontal ligament cells. There is many evidences that the some agents like cytokines and polypeptide growth factors are mediate these cellular events in wound healing. Recently someone is interested in herbal drugs on periodontal tissue healing processes. The purpose of this study was to examine the effects of 4 herbal drugs, Carthami Flis, Moutan Radicis Cortex, Scirpi Rhisoma, Seed of Carthamus tinctorius L. on human gingival fibroblasts and periodontal ligament cells. Periodontal ligament cells and gingival fibroblasts were primarily cultured from extracted premolar with non-periodontal diseases. The powder from extracted herbal drugs were prepared with distilled water. Cells were cultured with DMEM at 37degrees C, 5% CO2, 100% humidity incubator, and treated with each herbal drugs with proper concentration for 1, 2, and 3 days. The cell activity was determined by ELISA reader using MTT assay. There was the most significant elevation in 10(-3)g/ml of almost herbal drugs on cellular activities. The result of this study demonstrated that Carthami Flis, Moutan Radicis Cortex, Scirpi Rhisoma, Seed of Carthamus tinctorius L. appears to have beneficial effect on healing process after periodontal treatment.

MeSH Terms

Bicuspid
Carthamus tinctorius
Cytokines
Enzyme-Linked Immunosorbent Assay
Fibroblasts*
Humans
Humidity
Incubators
Intercellular Signaling Peptides and Proteins
Periodontal Ligament*
Water
Wound Healing
Cytokines
Intercellular Signaling Peptides and Proteins
Water