Korean J Fertil Steril.
2003 Jun;30(2):127-134.
Light and Electron Microscopic Observation in the Frozen-thawed Mouse Testicular Tissues
- Affiliations
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- 1Laboratory of Reproductive Biology and Infertility, Samsung Cheil Hospital & Women's Healthcare Center, Sungkyunkwan University School of Medicine, Seoul, Korea. sch-han@hanmail.net, arkangel@daum.net
- 2Department of Obstetrics & Gynecology, Samsung Cheil Hospital & Women's Healthcare Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
- 3Department of Life Science, College of Natural Science Soonchunhyang University, Asan, Choongnam, Korea.
Abstract
OBJECTIVE
The aim of this study was to investigate the morphological aspects of testicular tissue before and after freezing-thawing by light and transmission electron microscopy.
METHODS
Tissue biopsies were carried out on mouse testis for freezing. Samples in medium containing 20% glycerol were frozen by computer-controlled freezing program. The effect of freezing-thawing on the structural change of testicular tissues were examined by light and electron microscopy.
RESULTS
The freezing-thawing procedure had no significant effect on tubular diameter. However, it caused folding of the lamina propria, and notable damage to Sertoli cells, spermatogonia and spermatocytes. The cells were detached, desquamated from the basal lamina and had increased vacuolization. Round spermatids, elongated spermatids and spermatozoa were less affected, and most of them maintained their normal structure.
CONCLUSIONS
The structure of spermatogonia, spermatocyte and basal compartments in seminiferous epithelium was significantly altered by freezing-thawing procedure of mouse testicular tissues. Thus, we need to develop a more reliable method for the cryopreservation of testicular tissues.
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