Korean J Pathol.  2010 Jun;44(3):225-233.

Validation of Gene Expression Changes of Osteopontin and MMP-1 in Primary and Metastatic Colorectal Carcinomas

• Choi J
• Kim S
• Park JH
• Kim NK
• Kim H

Abstract

BACKGROUND
Metastasis is one of the most important characteristics of cancer in terms of its impact on patient survival. Unfortunately, identification of altered genes during tumor metastasis is limited.
METHODS
Using high-throughput microarrays containing 19K spotted human oligonucleotides, gene expression of primary and matched metastatic colon cancer were compared in previous study. Although DNA microarray analysis did not demonstrate complete classification of primary and metastatic carcinoma, 80 differentially expressed genes were identified. Among these, expression of osteopontin, matrix metalloproteinase-1 (MMP-1) and serpin A1 was assessed using immunohistochemistry in a validation set containing 43 pairs from tissue microarrays.
RESULTS
The expression of osteopontin was significantly higher in metastatic carcinoma than in primary carcinoma, as indicated by mRNA expression. The expression of MMP-1 was significantly lower in metastatic carcinoma. Expression of serpin A1 was not correlated with the microarray results.
CONCLUSIONS
Osteopontin and MMP-1 expression successfully classified primary and metastatic colorectal carcinomas and further studies on their clinical application is encouraged.

Keyword

Neoplasm metastasis; Colorectal neoplasms; Immunohistochemistry; Osteopontin; Matrix metalloproteinase 1

MeSH Terms

alpha 1-Antitrypsin
Colonic Neoplasms
Colorectal Neoplasms
Gene Expression
Humans
Immunohistochemistry
Matrix Metalloproteinase 1
Neoplasm Metastasis
Oligonucleotide Array Sequence Analysis
Oligonucleotides
Osteopontin
RNA, Messenger
Matrix Metalloproteinase 1
Oligonucleotides
Osteopontin
RNA, Messenger
alpha 1-Antitrypsin