J Korean Med Sci.  2013 Apr;28(4):593-601. 10.3346/jkms.2013.28.4.593.

Alpha Internexin Expression Related with Molecular Characteristics in Adult Glioblastoma and Oligodendroglioma

• Suh JH
• Park CK
• Park SH

• KMID: 1786969
• DOI: http://doi.org/10.3346/jkms.2013.28.4.593

Abstract

Alpha-internexin (INA) is a proneuronal gene-encoding neurofilament interacting protein. INA is overexpressed mostly in oligodendroglial phenotype gliomas, is related to 1p/19q codeletion, and is a favorable prognostic marker. We studied INA expression in oligodendrogliomas (ODGs) and glioblastomas (GBMs) to verify its association with several molecular phenotypes, 1p/19q codeletion, and epidermal growth-factor-receptor (EGFR) amplification. A total of 230 low- and high-grade ODG and GBM cases was analyzed for INA expression by immunohistochemical staining; and 1p/19q and EGFR gene status was examined by fluorescence in-situ hybridization. INA was positive in 80.3% of ODGs and in 34.3% of GBMs. 1p/19q codeletion was detected in 77.0% of ODGs and 5.5% of GBMs. INA and 1p/19q codeletion were strongly correlated (P < 0.001). The specificity of INA expression for 1p/19q codeletion was 70.8%, while sensitivity was 100%; positive predictive value was 72.5%, and negative predictive value was 29.2% in all 228 tumors. INA expression was correlated with better progression-free survival (PFS) and overall survival (OS) (P = 0.001). In conclusion, INA expression has high specificity and sensitivity to predict 1p/19q codeletion, and it is well correlated with PFS of both ODGs and GBMs. Therefore, INA expression could be a simple, reliable, and favorable prognostic and surrogate marker for 1p/19q codeletion and long term survival.

Keyword

Glioblastoma; Oligodendroglioma; Alpha-internexin, humans; 1p/19q Codeletion; EGFR protein, Human

MeSH Terms

Adult
Brain Neoplasms/*metabolism/mortality/pathology
Chromosomes, Human, Pair 1
Chromosomes, Human, Pair 19
Female
Gene Deletion
Glioblastoma/*metabolism/mortality/pathology
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence
Intermediate Filament Proteins/genetics/*metabolism
Kaplan-Meier Estimate
Male
Middle Aged
Oligodendroglioma/*metabolism/mortality/pathology
Phenotype
Predictive Value of Tests
Prognosis
Receptor, Epidermal Growth Factor/genetics/metabolism
Intermediate Filament Proteins
Receptor, Epidermal Growth Factor