J Periodontal Implant Sci.  2013 Feb;43(1):24-29. 10.5051/jpis.2013.43.1.24.

Correlation of expression and activity of matrix metalloproteinase-9 and -2 in human gingival cells of periodontitis patients

• Kim KA
• Chung SB
• Hawng EY
• Noh SH
• Song KH
• Kim HH
• Kim CH
• Park YG

• KMID: 1783674
• DOI: http://doi.org/10.5051/jpis.2013.43.1.24

Abstract

PURPOSE
Matrix metalloproteinases (MMPs) are capable of degrading extracellular matrix, and they are inducible enzymes depending on an inflammatory environment such as periodontitis and bacterial infection in periodontal tissue. Gingival inflammation has been postulated to be correlated with the production of MMP-2 and MMP-9. The objective of this study was to quantify the expression and activity of MMP-9 and -2, and to determine the correlation between activity and expression of these MMPs in human gingival tissues with periodontitis.
METHODS
The gingival tissues of 13 patients were homogenized in 500 microL of phosphate buffered saline with a protease inhibitor cocktail. The expression and activity of MMP-2 and -9 were measured by enzyme-linked immunosorbent assay and Western blot analysis, and quantified by a densitometer. For the correlation line, statistical analysis was performed using the Systat software package.
RESULTS
MMP-9 was highly expressed in all gingival tissue samples, whereas MMP-2 was underexpressed compared with MMP-9. MMP-9 activity increased together with the MMP-9 expression level, with a positive correlation (r=0.793, P=0.01). The correlation was not observed in MMP-2.
CONCLUSIONS
The expression of MMP-2 and -9 might contribute to periodontal physiological and pathological processes, and the degree of MMP-9 expression and activity are predictive indicators relevant to the progression of periodontitis.

Keyword

Matrix metalloproteinase 2; Matrix metalloproteinase 9; Periodontitis

MeSH Terms

Bacterial Infections
Blotting, Western
Enzyme-Linked Immunosorbent Assay
Extracellular Matrix
Humans
Inflammation
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Matrix Metalloproteinases
Pathologic Processes
Periodontitis
Protease Inhibitors

Figure

• 

  Figure 1 Expression of matrix metalloproteinase (MMP)-9 and -2 in patients with periodontitis. To analyze the expression of MMP-9 and -2, 100 µg of protein extracted from each of 13 samples was analyzed by Western blot. MMP-9, corresponding to molecular weight 94 kDa, and MMP-2, corresponding to molecular weight 72 kDa, were shown to be expressed in all of the samples. The numbers '1 to 13' indicate the number of each patient.

• 

  Figure 2 Matrix metalloproteinase (MMP)-2 and -9 gelatinolytic activity in patients with periodontitis. To analyze the activity of MMP-9 and -2, 10 µg of protein extracted from each of 13 samples was analyzed by gelatin zymography. Bands corresponding to MMP-9 and -2 can be observed in all samples. The numbers '1 to 13' indicate the number of each patient.

• 

  Figure 3 The correlation between the activity and expression of matrix metalloproteinase (MMP)-9 and -2 in patients with periodontitis. (A) Comparison of expression and activity of MMP-9 (upper panel) and -2 (lower panel) in individual patients. (B) Correlation between the activity and expression of MMP-9 and -2 in patients with periodontitis.

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