J Korean Med Sci.
2006 Apr;21(2):253-258. 10.3346/jkms.2006.21.2.253.
Prognostic Significance of Multidrug Resistance Gene 1 (MDR1), Multidrug Resistance-related Protein (MRP) and Lung Resistance Protein (LRP) mRNA Expression in Acute Leukemia
- Affiliations
-
- 1Department of Laboratory Medicine, College of Medicine, University of Ulsan and Asan Medical Center, Seoul, Korea. cjpark@amc.seoul.kr
- 2Department of Internal Medicine, College of Medicine, University of Ulsan and Asan Medical Center, Seoul, Korea.
- 3Department of Pediatrics, College of Medicine, University of Ulsan and Asan Medical Center, Seoul, Korea.
- 4Department of Laboratory Medicine, College of Medicine, Dogguk University, Seoul, Korea.
- KMID: 1781831
- DOI: http://doi.org/10.3346/jkms.2006.21.2.253
Abstract
- The prognostic significance of multidrug resistance (MDR) gene expression is controversial. We investigated whether multidrug resistance gene 1 (MDR1), multidrug resistance-related protein (MRP) and lung resistance protein (LRP) mRNA expression are associated with outcomes in acute leukemia patients. At diagnosis we examined MDR1, MRP and LRP mRNA expression in bone marrow samples from 71 acute leukemia patients (39 myeloid, 32 lymphoblastic) using nested RT-PCR. The expression of each of these genes was then expressed as a ratio in relation to beta-actin gene expression, and the three genes were categorized as being either 0, 1+, 2+ or 3+. MDR1, MRP and LRP mRNA expression was detected in 23.9%, 83.1% and 45.1 %, respectively. LRP mRNA expression was significantly associated with resistance to induction chemotherapy in acute leukemia patients, and in the AML proportion (p=0.02 and p=0.03, respectively). MRP and high MDR1 mRNA expression was associated with poorer 2-yr survival (p=0.049 and p=0.04, respectively). Patients expressing both MRP and LRP mRNA had poorer outcomes and had worse 2-yr survival. The present data suggest that MDR expression affects complete remission and survival rates in acute leukemia patients. Thus, determination of MDR gene expression at diagnosis appears likely to provide useful prognostic information for acute leukemia patients.
Keyword
MeSH Terms
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Vault Ribonucleoprotein Particles/*genetics
Survival Rate
RNA, Neoplasm/genetics
RNA, Messenger/genetics
Prognosis
Neoplasm Proteins/*genetics
Multidrug Resistance-Associated Proteins/*genetics
Middle Aged
Male
Leukemia, Myelocytic, Acute/drug therapy/genetics/mortality
Leukemia, Lymphocytic, Acute/drug therapy/genetics/mortality
Leukemia/drug therapy/*genetics/mortality
Infant
Humans
*Genes, MDR
Gene Expression
Female
Child, Preschool
Child
Base Sequence
Aged
Adult
Adolescent
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