J Korean Orthop Assoc.  2001 Feb;36(1):1-8.

Multidrug resistance-associated protein (MRP) and multidrug resistance (MDR1) gene expression in osteosarcoma and their prognostic significance

• Jung HG
• Lee SH
• Kim HS

Abstract

PURPOSE
The purpose of this study is to evaluate the correlations between the multidrug resistance-associated protein (MRP) and multidrug resistance (MDR1) gene in osteosarcoma, and their prognostic significances by RT-PCR method.
MATERIALS AND METHODS
We isolated RNA from 34 fresh deep-frozen primary osteosarcoma tissue specimens and evaluated the expression level of MRP and MDR1 m-RNA using RT-PCR method. GAPDH was used as the housekeeping gene for the experiment.
RESULTS
The average MRP/GAPDH m-RNA ratio was 0.550 and the average MDR1/GAPDH m-RNA ratio was 0.419. There were moderate degree of correlations between MRP/GAPDH m-RNA and MDR1/GAPDH m-RNA ratio (p<0.05). There was significant correlations between the chemotherapy response and the MDR1/GAPDH m-RNA ratio (p<0.05), but not with the MRP/GAPDH ratio. Multivariate analysis for the correlation between the MRP and MDR1 gene expression and the chemotherapy response revealed that only the MDR1/GAPDH m-RNA ratio was significantly related to it (p<0.05). CONCLUSION: There was a moderate degree of correlation between the expressions of MRP and MDR1 genes. However, the chemotherapy response showed significant correlations with the expression of MDR1 gene, but not with MRP gene.

Keyword

Osteosarcoma; Multidrug resistance-associated protein; Multidrug resistance gene; Chemotherapy response

MeSH Terms

Drug Resistance, Multiple*
Drug Therapy
Gene Expression*
Genes, Essential
Genes, MDR
Multidrug Resistance-Associated Proteins*
Multivariate Analysis
Osteosarcoma*
RNA
Multidrug Resistance-Associated Proteins
RNA