Yonsei Med J.
1995 Feb;36(1):15-25. 10.3349/ymj.1995.36.1.15.
Induction of ICAM-1 and HLA-DR expression by IFN-gamma in malignant melanoma cell lines
- Affiliations
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- 1Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.
- 2Laboratory of Biochemical Immunogenetics, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY, 10021, USA.
- KMID: 1729612
- DOI: http://doi.org/10.3349/ymj.1995.36.1.15
Abstract
- Two human malignant melanoma cell lines, Malme-3M and SK-Mel-28, were analyzed for their ability to induce the expression of intercellular adhesion molecule 1 (ICAM-1) and human leukocyte antigen (HLA)-DR molecules on their cell surfaces as well as at the transcriptional level before and after treatment with interferon (IFN)-gamma. Both cell lines demonstrated a high percentage(> 99%) of ICAM-1 expression regardless of IFN-gamma treatment. Before IFN-gamma treatment, Malme-3M cells barely expressed HLA-DR molecules (< 2%) and SK-Mel-28 cells demonstrated a relatively high percentage(> 50%) of HLA-DR expression. Both cell lines displayed elevated levels of HLA-DR expression in a time dependent manner after IFN-gamma treatment. However, these two cell lines have been shown to respond differentially to IFN-gamma. The molecular mechanism underlying such a differential behavior was investigated, and HLA-DR gene regulation was studied at the transcriptional level. Treatment with IFN-gamma led to the steady-state mRNA augmentation of the HLR-DR gene. The HLA-DRA mRNA augmentation was similar in both cell lines, whereas in Malme-3M, IFN-gamma did not augment the rate of transcription of the HLA-DRB gene as much as in SK-Mel-28. Data from this study established the fact that the melanoma cell lines displayed a differential susceptibility to IFN-gamma on the modulation of HLA-DR molecules, and this modulation was transcriptionally regulated.
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