J Vet Sci.  2009 Dec;10(4):361-363. 10.4142/jvs.2009.10.4.361.

Pathological features of bone marrow transplantation-related toxicity in a mouse

• Kim YH
• Ha CS
• Lee HS
• Lim SH
• Moon KS
• Chung MK
• Son HY

• KMID: 1726912
• DOI: http://doi.org/10.4142/jvs.2009.10.4.361

Abstract

In this case report, we present a mock-transduced bone marrow (BM) transplantation in a mouse, which was found moribund and autopsied to evaluate pathogenesis. Macroscopically, red discoloration of systemic organs was observed. Hematological values revealed a decrease in white blood cells, red blood cells, hematocrit, hemoglobin, and platelets, but an increase in reticulocytes. In BM cytology, hematopoietic cell lines were severely depleted. Histopathologically, hemorrhage in the cerebellar parenchyma, hemosiderin deposition and hemorrhage in the heart, necrosis and telangiectasia in liver, pulmonary parenchymal cysts, spermatogenic germ cells necrosis, atrophy and hemorrhage in testis, oligospermia and hemorrhage in the epididymis, and atrophy of BM, thymus and spleen were observed. In conclusion, autoimmune-like complications such as hematological value change, BM dysplasia and systemic hemorrhage appear to be the lethal cause of the mouse transplanted with mock-transduced BM.

Keyword

bone marrow transplantation; histopathology; pathogenesis; toxicity

MeSH Terms

Animals
Bone Marrow/pathology
Bone Marrow Transplantation/*adverse effects
Cerebellum/pathology
Hemorrhage/pathology
Liver/pathology
Lung/pathology
Male
Mice
Mice, Inbred C57BL
Myocardium/pathology
Testis/pathology
Thymus Gland/pathology

Figure

• 

  Fig. 1 Bone marrow (BM) cytology from the mouse found moribund 19 days after BM transplantation (BMT). BM cytology showed a severe loss of hematopoietic cells. (A) Few basophilic erythroblasts, basophils, macrophages, plasma cells, and lymphoblasts. (B) Proerythroblasts, polychromatic erythroblasts, and lymphocytes are present. Wright-Geimsa stain.

• 

  Fig. 2 Histopathological changes of the mouse found moribund 19 days after BMT. (A) Infiltration of large number of red blood cells is seen in the cerebellar parenchyma. (B) Cysts are seen in the pulmonary parenchyma. (C) Hemosiderin deposition is seen in heart myocytes. (D) Hepatocellular necrosis and dilated sinusoid are seen in the liver. (E, F) Hemorrhage in the testicular interstitial space, atrophy of seminiferous tubules, and necrosis of spermatogenic germ cells are noted. H&E stain.

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