J Korean Med Sci.  2007 Oct;22(5):773-778. 10.3346/jkms.2007.22.5.773.

Characteristics of Non-typhoidal Salmonella Isolates from Human and Broiler-chickens in Southwestern Seoul, Korea

• Cheong HJ
• Lee YJ
• Hwang IS
• Kee SY
• Cheong HW
• Song JY
• Kim JM
• Park YH
• Jung JH
• Kim WJ

• KMID: 1713279
• DOI: http://doi.org/10.3346/jkms.2007.22.5.773

Abstract

Non-typhoidal Salmonella (NTS) is an important commensal microorganism. The purpose of this study was to determine the epidemiological relation between NTS isolates from livestock and NTS isolates from human by analyzing antimicrobial susceptibilities and performing molecular typing. We determined the serotypes of 36 human clinical isolates and 64 livestock isolates, performed antimicrobial susceptibility testing against 8 antibiotics, and determined the molecular types of isolated NTS spp. by pulsed field gel electrophoresis (PFGE). In human isolates, S. enteritidis was the most common serotype (17 isolates; 47.2%) and S. typhimurium the second most (8 isolates; 22.2%). In livestock isolates, S. typhimurium was the most common serotype (15 isolates; 23.44%), and S. enteritidis was the second most (14 isolates; 21.88%). Ampicillin and tetracycline resistance were 50% (32/64 isolates) each among broiler-chicken NTS isolates. No human or livestock NTS isolates showed resistance to ciprofloxacin, TMP-SMX, or ceftriaxone. However, 19.4% (7/36) and 46.8% (30/64) of the human and livestock NTS isolates were resistant to nalidixic acid (MIC > or =16 mg/mL), respectively. The presence of the three identical PFGE molecular types from human and broiler-chicken NTS isolates suggests the possibility of transmission from livestock to humans.

Keyword

Salmonella Infections; Salmonella Enteritidis; Salmonella Typhimurium; Epidemiology

MeSH Terms

Adult
Animals
Chickens
Cluster Analysis
Drug Resistance, Bacterial
Female
Humans
Korea
Male
Nalidixic Acid/pharmacology
Salmonella Infections/epidemiology/metabolism/*microbiology
Salmonella Infections, Animal/epidemiology/metabolism/*microbiology
Salmonella enteritidis/metabolism
Salmonella typhimurium/*metabolism
Serotyping

Figure

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  Fig. 1 Locations of study hospital (H) and three slaughter-houses (S) in the southwest area of Seoul and its suburbs.

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  Fig. 2 Antimicrobial resistance (%) of human and broiler-chicken NTS isolates.

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  Fig. 3 Dendrogram showing cluster analysis of PFGE Xba-I patterns from 54 strains of S. enteritidis and S. typhimurium generated with GelCompar by the UPGMA method. The different PFPs and number of strains tested are indicated. (A) S. enteritidis, (B) S. typhimurium.

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