J Vet Sci.  2007 Jun;8(2):155-161. 10.4142/jvs.2007.8.2.155.

Dissemination and tracking of Salmonella spp. in integrated broiler operation

• Kim A
• Lee YJ
• Kang MS
• Kwag SI
• Cho JK

• KMID: 1089667
• DOI: http://doi.org/10.4142/jvs.2007.8.2.155

Abstract

Controlling Salmonella in integrated broiler operation is complicated because there are numerous potential sources of Salmonella contamination, including chicks, feed, rodents, wild poultry operations, and the processing plant. The objective of this study was to investigate the distribution of Salmonella through all phases of two integrated broiler operations and to determine the key areas related to the control of all known sources of infection. Two different Salmonella serotypes were observed at integrated broiler chicken company A. S. enteritidis, the predominant company A isolate, was consistently found in the breeder farm, hatcheries, broiler farms, and chicken slaughterhouse. At company B, a total of six different serotypes, S. heidelberg, S. senftenberg, S. enteritidis, S. blockley, S. gallinarum, and S. virchow, were detected. Although S. heidelberg was not found in the broiler farms, it was consistently found in the breeder farm, hatcheries, and chicken slaughterhouse. In addition, S. enteritidis was found in the hatcheries, broiler farm, and chicken slaughterhouse. In order to obtain the genetic clonality, 22 S. enteritidis isolates were digested with XbaI and analyzed by pulsed-field gel electrohporesis (PFGE). A difference in the PFGE pattern was found to be related to the origin of the integrated broiler operation. These data support the critical need to control Salmonella in breeder farms and hatcheries, and demonstrate important points related to the control of infection in large-scale poultry operations of Korea.

Keyword

broiler; operation; Salmonella spp. slaughterhouse

MeSH Terms

Animal Husbandry
Animals
*Chickens
DNA, Bacterial/chemistry/genetics
Electrophoresis, Gel, Pulsed-Field/veterinary
Female
Food Microbiology
Korea/epidemiology
Poultry Diseases/epidemiology/*microbiology/transmission
Salmonella/classification/genetics/*isolation & purification
Salmonella Infections, Animal/epidemiology/*microbiology/transmission

Figure

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  Fig. 1 Transmission of Salmonella in the integrated broiler chicken companies. (A) The results for integrated broiler chicken company A. (B) The results for integrated broiler chicken company B.

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  Fig. 2 Pulsed field gel electrophoresis patterns of S. enteritidis isolates obtained with the XbaI restriction enzyme. M: Lambda ladder marker for PFGE; Lane 1 to 11: S. enteritidis isolated from integrated broiler company A; Lane 12 to 22: S. enteritidis isolated from integrated broiler company B.

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