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Establishment and Application of a Multibead Serotyping Assay for Pneumococci in Korea

Kim HW, Lee S, Lee M, Kim KH

PURPOSE: Serotyping pneumococcal isolates is important to monitor efficacy of pneumococcal vaccines. Because of difficulties of typing pnueumocci, a multiplex bead-based (multibead) serotyping assay was recently introduced. The aim of...
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Serodiagnosis and Serotyping of Chlamydia Trachomatis in Male Urogenital Organ with Indirect microimmunofluorescence Technique

Kim SJ, Lee MS

  • KMID: 2289781
  • Korean J Urol.
  • 1985 Apr;26(2):102-108.
Laboratory techniques for diagnosis of C. trachomatis infection are of increasing interest and classified as (1) isolation of the organisms (2) direct demonstration of the organisms and (3) detection of...
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Modified Method of Multilocus Sequence Typing (MLST) for Serotyping in Salmonella Species

Yun YS, Chae SJ, Na HY, Chung GT, Yoo CK, Lee DY

Multilocus sequence typing (MLST) of Salmonella is useful method for replacing serotyping using antisera but is limited by difficulties associated with in polymerase chain reaction (PCR). We optimized the PCR...
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Isolation, Serotyping and Nucleotide Sequence Analysis of Bovine Ratavirus Isolated from Korean Native Cattle

Yu JH, Cha KJ, Kim ER, Kim YS, Lee YK, Song JO, Cho HC, Ju JS, Park BS, Yoo DH, Kim SM, Ji BJ, Lee JB, Urasawa S, Koki T, Greenberg

  • KMID: 1561238
  • J Korean Soc Virol.
  • 2000 Sep;30(3):189-202.
No Abstract Available.
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Serotyping of Chlamydia trachomatis by Polymerase Chain Reaction: Restriction Fragment Length Polymorphism Analysis

Seo YH, Park PW, Kim W, Kim DA, Choi TY

  • KMID: 2225965
  • Korean J Clin Pathol.
  • 2000 Oct;20(5):480-485.
BACKGROUND: Chlamydia trachomatis is currently classified into 15 serovars (A, B, Ba, C, D, E, F, G, H, I, J, K, L1, L2, L3) and a large number of serovariants....
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Evaluation of serotyping using monoclonal antibodies and PCR-RFLP for Chlamydia trachomatis serotype identification

Choi TY, Kim DA, Seo YH

We compared genotyping by restriction fragment length polymorphism (RFLP) analysis of the amplified omp1 gene with serotyping by dot enzyme-linked immunosorbent assay (dot-ELISA) to determine the suitability of RFLP analysis...
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Isolation of Cryptococcus neoformans var. grubii (serotype A) from Pigeon Droppings in Korea

Chee HY, Kim YK

Three hundred and sixty five samples of avian droppings, collected from parks and zoo, were investigated for the occurrence of Cryptococcus neoformans in Korea. Thirteen samples were positive for C....
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Comparison of Serotypes of Group A Streptococci between Seoul and Chinju

Jung HJ, Kim SJ, Kim YJ, Maeng KY

  • KMID: 2300533
  • Korean J Clin Pathol.
  • 1998 Dec;18(4):565-569.
BACKGROUND: To interpret the serotyping data from group A streptococcal infections and their complications, serotyping data from healthy children are essential. In this study, we determined the serotypes of the...
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Epidemiologic Characteristics of T Serotyping in Relation to the Outbreak of Erythromycin Resistant Streptocccus pyogenes

Cha SH, Hwang KJ, Lee YH

  • KMID: 1967590
  • J Korean Pediatr Soc.
  • 2000 Oct;43(10):1323-1329.
PURPOSE: The identification of antigenic specificity of Streptococcus pyogenes using T serotyping is important to understand biologic characteristics of microorganisrns. We would like to disover the association of the occurrence of predominant...
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A study of serotyping of Streptococcus pneumoniae by multibead assay

Cho KY, Lee JA, Cho SE, Kim NH, Lee JA, Hong KS, Lee HJ, Kim KH

PURPOSE: Streptococcus pneumoniae is a major etiologic agent for pneumonia, meningitis, otitis media, and sepsis among young children. Multi-drug resistant strains have raised great concern worldwide, thus the importance of...
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Comparison of Multiplex Latex Bead Flow Cytometric Analysis and Quellung Analysis in Serotyping Pneumococci

Choi KM, Yeon SI, Kim ES, Shin JS, Yong DE, Lee KW, Kim DS

PURPOSE: The serotyping results of the Quellung reaction was compared with the newly developed multiplex latex assay and the sensitivity of the Quellung reaction was compared. METHODS: We checked the serotypes...
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Epidemiological Studies of Yersinia pseudotuberculosis Infection by Plasmid DNA Profile Assay

Paik IK, Cho CR, Kim MA, Fukushima

  • KMID: 2082862
  • Korean J Infect Dis.
  • 1997 Jan;29(1):33-37.
BACKGROUND: In order to investigate transmission route of Yersinia pseudotuberculosis infection in Korea, we tried epidemiological study among human strains, mountain spring water strain and wild mouse strain which were...
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Seasonal Distribution of T Serotyping and emm Genotyping of Group A Streptococci Obtained from Children with Streptococcal Infections in Masan, Korea, 2003~2004

Jeon HS, Park HJ, Lee HJ, Ma SH, Cha SH

PURPOSE: The aim of this study is to know seasonal distribution of group A streptococci obtained from one center using emm genotyping and T serotyping in Masan from 2003 through...
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Molecular Serotyping of Group B Streptococcus Isolated from the Pregnant Women by Polymerase Chain Reaction and Sequence Analysis

Oh CE, Jang HO, Kim NH, Lee J, Choi EH, Lee HJ

  • KMID: 2278823
  • Korean J Pediatr Infect Dis.
  • 2009 Jun;16(1):47-53.
PURPOSE:This study was performed to investigate the serotype distribution of group B streptococcus (GBS) isolated from pregnant Korean women using molecular methods. METHODS:The study materials included 42 GBS isolates obtained from...
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Streptococcus pneumoniae Type Determination by Multiplex Polymerase Chain Reaction

Yun KW, Cho EY, Hong KB, Choi EH, Lee HJ

The purpose of this study was to develop pneumococcal typing by multiplex PCR and compare it with conventional serotyping by quellung reaction. Pneumococcal strains used in this study included 77...
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Serotyping and Phylogenetic analysis of Enteroviruses Isolated from Patients with Aspetic Meningitis

Lee JH, Ahn BY, Ban SH, Kim SH, Kim EC

  • KMID: 1892743
  • Korean J Clin Microbiol.
  • 2000 Sep;3(2):121-131.
BACKGROUND: The determination of serotype of enteroviruses is useful for the discrimination between sporadic and epidemic infections. The conventional serotyping method is time-consuming and labor-intensive. Recently, molecular method was introduced...
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The Bacteriological Survey of the Main Streams in Kyong-Ju City: on Samonellae

Che IH

  • KMID: 1983466
  • Korean J Prev Med.
  • 1989 Jun;22(2):268-275.
The studies on the standard plate count, most probable number(MPN) and isolation of the Enterobacteriaceae, especially Salmonella species from stream waters of Kyong-Ju city were carried out from June to...
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Serotyping of Group A Streptococci Isolated from Healthy School Children and Patients with Pharyngotonsillitis

Cha S, Park Y, Suh J, Johnson D

  • KMID: 1557266
  • Korean J Infect Dis.
  • 1998 Feb;30(1):19-23.
BACKGROUND: To evaluate serological typing of T(epidemiologic marker) and M protein(major virulence antigen) is important to understand pathogenesis and epidemiology of streptococcal infection. The purpose of this study is to...
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Rh C/c , E/e genotyping by polymerase chain reaction using sequence - specific primers

Hwang YS, Oh HB, Kim SI, Seo DH

  • KMID: 1838435
  • J Korean Soc Microbiol.
  • 1999 Feb;34(1):21-26.
BACKGROUNDS: With the recent elucidation of genetic basis of Rh blood group, it is now available the molecular genotyping methods for Rh blood typing. These can be used when serological...
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The Multiplex RT-PCR for the Serotyping of Hantaviruses

Lee PW, Park MS

  • KMID: 2099294
  • J Korean Soc Microbiol.
  • 1998 Oct;33(5):451-461.
Hantaviruses are negative-strand RNA viruses that contain three segmented genome and belong to the family Bunyaviridae. Due to such an unique structure of segmented RNA genome, these viruses have a...
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