J Vet Sci.
2013 Dec;14(4):481-486. 10.4142/jvs.2013.14.4.481.
Evaluation of a side population of canine lymphoma cells using Hoechst 33342 dye
- Affiliations
-
- 1The Laboratory of Clinical Pathology and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. yongbaek@snu.ac.kr
- 2Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA.
- 3Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA.
- KMID: 1712316
- DOI: http://doi.org/10.4142/jvs.2013.14.4.481
Abstract
- Cancer stem cell (CSC) research has increased exponentially to gain further insight into the mechanisms underlying both carcinogenesis and chemotherapy resistance. The present study was performed to explore the potential value of a side population (SP) assay for identifying and characterizing putative CSCs among canine lymphoma cells. Canine lymphoma cells from cell lines and clinical samples were subjected to the SP assay consisting of Hoechst 33342 staining and subsequent flow cytometric analysis. The SP assay revealed various amounts of a SP fraction among the canine lymphoma cells. The percentages of SP were not affected by inhibitors of membrane transporters, verapamil hydrochloride, or fumitremorgin C. Most of the canine lymphoma cells expressed high levels of Bmi-1 and membrane transporter proteins such as ABCG2 and phosphorylated (p)-glycoprotein. This investigation lays the groundwork for further studies of the biological behaviors and molecular characteristics of CSCs in cases of canine lymphoma.
Keyword
MeSH Terms
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Animals
Benzimidazoles/*metabolism
Cell Line, Tumor
Dog Diseases/*diagnosis/drug therapy/pathology
Dogs
Flow Cytometry/*methods/veterinary
Fluorescent Dyes/*metabolism
Gene Expression Regulation, Developmental
Lymphoma/diagnosis/drug therapy/pathology/*veterinary
Neoplastic Stem Cells/drug effects/*metabolism/pathology
Side-Population Cells/drug effects/*metabolism/pathology
Benzimidazoles
Fluorescent Dyes
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