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Monitoring Glutathione Dynamics and Heterogeneity in Living Stem Cells

Jeong EM, Shin JW, Lim J, Kim JH, Kang H, Yin Y, Kim HM, Kim Y, Kim SG, Kang HS, Shin DM, Choi K, Kim IG

Glutathione (GSH) is a major antioxidant in cells, and plays vital roles in the cellular defense against oxidants and in the regulation of redox signals. In a previous report, we...
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Indocyanine Green-Fluorescent Pancreatic Perfusion-Guided Resection of Distal Pancreas in Solid Pseudopapillary Neoplasm: Usefulness and Feasibility During Pancreaticobiliary Surgery

Kim SH, Rho SY, Kang CM

ICG is widely applied in real-time imaging during abdominal surgery, plastic surgery, as well as oncologic staging and treatment. A twenty-eight year-old female patient was found to have a 4.5...
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Development of Molecular Diagnosis Using Multiplex Real-Time PCR and T4 Phage Internal Control to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis from Human Stool Samples

Shin JH, Lee SE, Kim TS, Ma DW, Cho SH, Chai JY, Shin EH

This study aimed to develop a new multiplex real-time PCR detection method for 3 species of waterborne protozoan parasites (Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis) identified as major causes...
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Structure-Inherent Targeting of Near-Infrared Fluorophores for Image-Guided Surgery

Jo D, Hyun H

Although various clinical imaging modalities have been developed to visualize internal body structures and detect abnormal tissues prior to surgical procedures, most medical imaging modalities do not provide disease-specific images...
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NIRF Heptamethine Cyanine Dye Nanocomplexes for Multi Modal Theranosis of Tumors

Thomas RG, Jeong YY

Heptamethine cyanine dyes are categorized as a class of near infrared fluorescent (NIRF) dyes which have been discovered to have tumor targeting and accumulation capability. This unique feature of NIRF...
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Optical Tools to Investigate Cellular Activity in the Intestinal Wall

Boesmans W, Hao MM, Berghe PV

  • KMID: 2433473
  • J Neurogastroenterol Motil.
  • 2015 Jul;21(3):337-351.
  • doi: 10.5056/jnm15096
Live imaging has become an essential tool to investigate the coordinated activity and output of cellular networks. Within the last decade, 2 Nobel prizes have been awarded to recognize innovations...
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Current Status of Optical Imaging for Evaluating Lymph Nodes and Lymphatic System

Lee ES, Kim TS, Kim SK

Optical imaging techniques use visual and near infrared rays. Despite their considerably poor penetration depth, they are widely used due to their safe and intuitive properties and potential for intraoperative...
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Optimization of ultrasound parameters for microbubble-nanoliposome complex-mediated delivery

Yoon YI, Yoon TJ, Lee HJ

PURPOSE: The aim of this study was to identify the optimal ultrasound (US) parameters for gene and drug delivery. METHODS: In order to target SkBr3, which is a breast cancer cell...
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In-vivo Optical Measurement of Neural Activity in the Brain

Kim SA, Jun SB

The optical neural recording techniques are promising tools in recent years. Compared to the traditional electrophysiological recording, the optical means offer several advantages including no inclusion of electrical noise, simultaneous...
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Multiparameter Flow Cytometry: Advances in High Resolution Analysis

O'Donnell , Ernst DN, Hingorani

Over the past 40 years, flow cytometry has emerged as a leading, application-rich technology that supports high-resolution characterization of individual cells which function in complex cellular networks such as the...
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Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis

Garcia A, Martinez R, Benitez-Medina JM, Risco D, Garcia WL, Rey J, Alonso JM, de Mendoza JH

Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green...
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Evaluation of a side population of canine lymphoma cells using Hoechst 33342 dye

Kim MC, D'Costa S, Suter S, Kim Y

Cancer stem cell (CSC) research has increased exponentially to gain further insight into the mechanisms underlying both carcinogenesis and chemotherapy resistance. The present study was performed to explore the potential...
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Distribution and accumulation of Cy5.5-labeled thermally cross-linked superparamagnetic iron oxide nanoparticles in the tissues of ICR mice

Hue JJ, Lee HJ, Jon S, Nam SY, Yun YW, Kim JS, Lee BJ

Free Cy5.5 dye and Cy5.5-labeled thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION) have been routinely used for in vivo optical imaging. However, there is little information about the distribution and...
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Improved assessment of frozen/thawed mouse spermatozoa using fluorescence microscopy

Diercks AK, Burgers HF, Schwab A, Schenkel J

Genetically modified (GM) animals are unique mutants with an enormous scientific potential. Cryopreservation of pre-implantation embryos or spermatozoa is a common approach for protecting these lines from being lost or...
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Antioxidant activities of licorice-derived prenylflavonoids

Kim HJ, Seo JY, Suh HJ, Lim SS, Kim JS

  • KMID: 2313633
  • Nutr Res Pract.
  • 2012 Dec;6(6):491-498.
Glycyrrhiza uralensis (or licorice) is a widely used Oriental herbal medicine from which the phenylflavonoids dehydroglyasperin C (DGC), dehydroglyasperin D (DGD), and isoangustone A (IsoA) are derived. The purpose of...
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Quantitative Determination of Plasmodium Parasitemia by Flow Cytometry and Microscopy

Jun G, Lee JS, Jung YJ, Park JW

The traditional light microscopy has limitations for precise growth assays of malaria parasites in culture or for assessment of new compounds for antimalarial activity; the speed and high reproducibility of...
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Molecular Imaging of Gastrointestinal Malignancies

Kim JW, Yoon SM, Myung SJ

Endoscopic detection of gastrointestinal cancer currently depends in large part on white light images, which show mucosal surface changes in real time. Molecular imaging is a rapidly growing new field...
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Comparative Quantitative Analysis of Cluster of Differentiation 45 Antigen Expression on Lymphocyte Subsets

Im M, Chae H, Kim T, Park HH, Lim J, Oh EJ, Kim Y, Park YJ, Han K

BACKGROUND: Since the recent introduction of radioimmunotherapy (RIT) using antibodies against cluster of differentiation (CD) 45 for the treatment of lymphoma, the clinical significance of the CD45 antigen has been...
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Relationship between sperm quality traits and field-fertility of porcine semen

Tsakmakidis IA, Lymberopoulos AG, Khalifa TA

An investigation involving seven boars, active in artificial insemination, and 1,350 multiparous sows was conducted at a private farm and aimed at examining the relationship between sperm quality traits and...
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Preliminary Effects of Oral Uridine on the Ocular Surface in Dry Eye Patients

Chang KC, Oh JY, In YS, Kim MK, Shin KC, Wee WR, Lee JH, Park MG

We designed a randomized, double blinded, 3-months controlled prospective clinical study to investigate effects of oral uridine on the ocular surface in dry eye patients. Twenty-seven patients who diagnosed as...
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