Korean J Gynecol Oncol Colposc.  1994 Sep;5(3):29-38.

Detection of Cytokine Gene Expression in Ovarian Cancer Tissues

• Jeong SH
• Chun SS
• Cho YL
• Lee TH
• Jeong YJ
• Kim HJ
• Kim JC

Abstract

The variable clinical responses seen with rnost cancer immunotherapy suggests that there is a large interindividual variation in immunoiogic response to tumors. One of the key functional parameter.s of an immune response is the loca1 production of cytokines. These multifunctional cytokines have been implicated in growth stimulation and cytotoxicity of ovarian tumor celle. To determine the presence and quantities of cytokines in ovarian cancer tissue, the cons-titutive expression of cytokine mRNA in biopsies from epithelial ovarian carcinomas was determined by using a PCR-assisted mRNA amplification asssy(RT-PCR). Using a set of cytokine-specific primers for 14 different cytokines, distinctive patterns of cytokine gene expression between normal and malignant ovarian tissues could be found. Several cytokines were detected even in the norma1 ovarian tissue including IL-1A, IL-8, TGF-A, TGF-B, GM-CSF, and IL,-2R. In case of the ovarian cancer, the signals were more intense than normal and we could detect the expression of some other cytokines, such as IL-1B, IL- 4, IL-6, IL-l0, TNF, and INF-T, which could not be detected in normal tissue. The presence of eytokines in ovarian cancer tissue could be impartant in the growth and development of cancer, more specifically, in relation to host immune responsiveness.

MeSH Terms

Biopsy
Cytokines
Gene Expression*
Granulocyte-Macrophage Colony-Stimulating Factor
Growth and Development
Immunotherapy
Interleukin-6
Interleukin-8
Ovarian Neoplasms*
RNA, Messenger
Cytokines
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-6
Interleukin-8
RNA, Messenger