Korean J Physiol Pharmacol.  2001 Dec;5(6):451-456.

Effect of kainic acid on the phosphorylation of mitogen activated protein kinases in rat hippocampus

• Won JS
• Lee JK
• Choi SS
• Song DK
• Huh SO
• Kim YH
• Suh HW

Abstract

In rat hippocampus, kainic acid (KA; 10 mg/kg; i.p.) increased the phosphorylated forms of ERK1/2 (p-ERK1/2) and Jun kinase1 (p-JNK1), but not p-JNK2 and p38 (p-p38). The preadministration with cycloheximide (CHX; 5 mg/kg; i.p.) inhibited KA-induced increase of p-JNK1, but not p-ERK1/2. Surprisingly, the phosphorylated upstream MAP kinase kinases (p-MKKs) were not correlated with their downstream MAP kinases. The basal p-MKK1/2 levels were completely abolished by KA, which were reversed by CHX. In addition, p-MKK4 and p-MKK3/6 levels were enhanced by CHX alone, but were attenuated by KA. Thus, our results showed that KA increased the p-ERK and p-JNK levels in rat hippocampus, which were not parallel with their classical upstreamal kinases.

MeSH Terms

Animals
Cycloheximide
Hippocampus*
Kainic Acid*
Mitogen-Activated Protein Kinase Kinases
Mitogen-Activated Protein Kinases*
Phosphorylation*
Phosphotransferases
Rats*
Cycloheximide
Kainic Acid
Mitogen-Activated Protein Kinase Kinases
Mitogen-Activated Protein Kinases
Phosphotransferases