J Clin Pathol Qual Control.  2000 Jun;22(1):175-180.

Mathematical Correction of Hemolysis Interference with Hemolysis Index

• Won DI
• Jin HK
• Yoon KJ

Abstract

BACKGROUND: We studied the expected hemoglobin concentration and mathematical correction with hemolysis index in determinations of aspartate aminotransferase (AST), creatine kinase (CK) and lactate dehydrogenase (LD) on which the effect of in vitro hemolysis has been known to be relatively large.
METHODS
On the Hitachi 747 hemoglobin was measured bichromatically. In hemolysis interference studies by hemolysates hemolysis index and hemoglobin were determined as well as AST, CK and LD. Obtained correction formulas were applied for hemolysed samples. Mathematically corrected values were compared with resampled values.
RESULTS
Hemoglobin concentration could be calculated as a function of hemolysis index. Corrected values were correlated better with resampled values than uncorrected values. Two % of referred serum samples had hemoglobin concentration more than 100 mg/dL.
CONCLUSIONS
Hemolysis interference in serum samples was found to be frequent and mathematical correction with hemolysis index could be helpful for AST, CK and LD.

MeSH Terms

Aspartate Aminotransferases
Creatine Kinase
Hemolysis*
L-Lactate Dehydrogenase
Aspartate Aminotransferases
Creatine Kinase
L-Lactate Dehydrogenase