Immune Netw.  2013 Oct;13(5):218-221. 10.4110/in.2013.13.5.218.

B Cells Promote Th1- Skewed NKT Cell Response by CD1d-TCR Interaction

• Shin JH
• Park SH

• KMID: 1493617
• DOI: http://doi.org/10.4110/in.2013.13.5.218

Abstract

CD1d expressing dendritic cells (DCs) are good glyco-lipid antigen presenting cells for NKT cells. However, resting B cells are very weak stimulators for NKT cells. Although alpha-galactosylceramide (alpha-GalCer) loaded B cells can activate NKT cells, it is not well defined whether B cells interfere NKT cell stimulating activity of DCs. Unexpectedly, we found in this study that B cells can promote Th1-skewed NKT cell response, which means a increased level of IFN-gamma by NKT cells, concomitant with a decreased level of IL-4, in the circumstance of co-culture of DCs and B Cells. Remarkably, the response promoted by B cells was dependent on CD1d expression of B cells.

Keyword

B cells; Dendritic cells; NKT cells; alpha-galactosylceramide and Th1 response

MeSH Terms

Antigen-Presenting Cells
B-Lymphocytes*
Coculture Techniques
Dendritic Cells
Galactosylceramides
Interleukin-4
Natural Killer T-Cells*
Galactosylceramides
Interleukin-4

Figure

• 

  Figure 1 Effects of αGalCer pulsed B cells on NKT cells activation. DCs and B cells were pulsed for 4 h with 10 ng/ml α-GalCer, and then the excess α-GalCer was removed by washing. Splenic NKT cells were co-cultured with α-GalCer pulsed DCs and B cells that were mixed at indicated ratio (10~30% of number of NKT cells) and the production of IFN-γ and IL-4 was determined after 48 h (A), (B) or 8 h (C) using ELISA (A), (B) and intracellular staining (C). The results shown in (C) are representative of NK1.1+ TCRβ+ cells, and the results shown in (A), (B) were expressed as the mean±SD of triplicate measurements. The data are representative of three independent experiments. ND, not determined. *p<0.1, **p<0.01 and ***p<0.001.

• 

  Figure 2 Effects of αGalCer un-pulsed B cells on NKT cells activation. DCs and were pulsed for 4 h with 10 ng/ml α-GalCer, and then the excess α-GalCer was removed by washing. Splenic NKT cells were co-cultured with α-GalCer pulsed DCs and α-GalCer un-pulsed B cells that were mixed at indicated ratio (10~30% of number of NKT cells) and production of IFN-γ (A) and IL-4 (B) was determined after 48 h by using ELISA. The results were expressed as the mean±SD of triplicate measurements. The data are representative of three independent experiments. ND, not determined. *p<0.1.

• 

  Figure 3 Effects of CD1d expressing B cells on NKT cells activation. B cells were purified from CD1d+/- or CD1d-/- mice respectively. DCs and B cells were pulsed for 4 h with 10 ng/ml α-GalCer, and then the excess α-GalCer was removed by washing. Splenic NKT cells were co-cultured with α-GalCer pulsed DCs and B cells and then production of IFN-γ (A) and IL-4 (B) was determined after 48 h by using ELISA. The results were expressed as the mean±SD of triplicate measurements. The data are representative of three independent experiments. **p<0.01 and ***p<0.001.

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