Korean J Med Mycol.
2009 Mar;14(1):9-15.
Protective Effects of Immunization with Proteinase, Phospholipase and Cell Wall Proteins of Candida albicans in Mice Candidosis
- Affiliations
-
- 1Department of Microbiology, Kwandong University College of Medicine, Kangnung, Korea.
- 2Department of Microbiology, Yonsei University Wonju College of Medicine and Institute of Basic Medicine, Yonsei University, Wonju, Korea. leekh@yonsei.ac.kr
- 3Department of Anatomy, Yonsei University Wonju College of Medicine, Yonsei University, Wonju, Korea.
Abstract
- BACKGROUND
The opportunistic fungus Candida albicans is a major pathogen especially to immunocompromised patients.
OBJECTIVES
We examined the protective effect of the active and passive immunizations to evaluate the applicability for the treatment of candidosis in Candida-infected mice model.
METHODS
Candida cell wall components were obtained by treatment of lyticase, proteinase K, and dithiothreitol. The proteinase was purified from the culture filtrates of C. albicans using a series of chromatographic steps consisting of DEAE-Sepharose FF, Sephacryl S-200 HR and size-exclusion high performance liquid chromatography. The phospholipase was purified from the culture supernatant of C. albicans with DEAE column chromatography, reverse phase column chromatography, revere phase HPLC and size-exclusion HPLC. Antibodies to cell wall protein components, proteinase and phospholipase were produced by immunization into mice of same strain.
RESULTS
The mean survival times of active and passive immunized mice groups were longer than those of non-immunized groups.
CONCLUSION
These results showed that immunization with proteinase and its antibody were the most effective to prolong survival time in Candida-infected mice.
Keyword
MeSH Terms
-
Acrylic Resins
Animals
Antibodies
Candida
Candida albicans
Cell Wall
Chromatography
Chromatography, High Pressure Liquid
Chromatography, Liquid
Chromatography, Reverse-Phase
Dithiothreitol
Endopeptidase K
Ethanolamines
Fungi
Glucan Endo-1,3-beta-D-Glucosidase
Immunization
Immunization, Passive
Mice
Multienzyme Complexes
Peptide Hydrolases
Phospholipases
Proteins
Survival Rate
Acrylic Resins
Antibodies
Dithiothreitol
Endopeptidase K
Ethanolamines
Glucan Endo-1,3-beta-D-Glucosidase
Multienzyme Complexes
Peptide Hydrolases
Phospholipases
Proteins
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Seroreactivities of proteinases of Candida albicans, C. tropicalis, and C. parapsilosis in sera from various Candida species-infected mice
- Serum Indeces Protein Fibrils Expression in Candida albicans
- Effects of Culture Condition on Secretion of Phospholipase from Candida albicans
- Optimum Culture Conditions for Production of Proteinases secreted by Candida albicans , C. tropicalis , and C. parapsilosis
- Detection of Antibody to Candida albicans Proteinase in Sera from C. albicans - Cultured Patients and Healthy Controls
