Korean J Med Mycol.  1997 Dec;2(2):123-128.

Effects of Culture Condition on Secretion of Phospholipase from Candida albicans

• Shin WS
• Lee KH
• Park JY
• Koh CM

Abstract

BACKGROUND: The dimorphic yeast, Candida albicans, is considered as a dangerous opportunistic pathogen in immunocompromised hosts. Several phospholipases of C. albicans are known to be secreted into the culture medium. Phospholipases have been proposed as a virulence factor in the pathogenesis of Candida infections.
OBJECTIVE
In order to investigate enzyme production, we examined culture condition of secreted phospholipase production from C. albicans.
METHODS
C. albicans ATCC 10231 was cultivated in various media at 37 degrees C for 3 days. Phospholipase activity was measured by fatty acid soap precipitation in plate containing 0.04% lecithin, 0.1 M citrate buffer, pH 4.2 and 1.5% noble agar.
RESULTS
Phospholipase was highly induced when C. albicans was cultivated in broth medium (containing glucose 2%, albumin 0.2% and Fe++ ion 0.01%) and Saboulaud's dextrose agar supplemented with 0.01% sodium deoxycholate.
CONCLUSION
Highly induction of secreted phospholipase by albumin from C albicans may be play an important role in tissue invasion in the pathogenesis of C. albicans.

Keyword

Candida; albicans; Phospholipase; Production

MeSH Terms

Agar
Candida albicans*
Candida*
Citric Acid
Deoxycholic Acid
Glucose
Hydrogen-Ion Concentration
Immunocompromised Host
Lecithins
Phospholipases*
Soaps
Virulence
Yeasts
Agar
Citric Acid
Deoxycholic Acid
Glucose
Lecithins
Phospholipases
Soaps