Anat Cell Biol.  2010 Dec;43(4):303-309. 10.5115/acb.2010.43.4.303.

Downregulation of NFAT2 promotes melanogenesis in B16 melanoma cells

Affiliations
  • 1Department of Anatomy, School of Medicine, Chungnam National University, Daejeon, Korea. yhlee@cnu.ac.kr
  • 2Department of Dermatology, School of Medicine, Chungnam National University, Daejeon, Korea.
  • 3Department of Physiology, School of Medicine, Chungnam National University, Daejeon, Korea.

Abstract

Nuclear factor of activated T-cells (NFAT) proteins are, calcium-regulated transcription factors, key regulator of stimulation-dependent gene activation. In our microarray analysis for the genes expressed in human black and white hairs, NFAT2 was significantly upregulated in the white hair, compared to the black hair. The aim of this study was to investigate functional role of NFAT2 in melanogenesis. Western blot analysis was performed to investigate the expression of NFAT2 protein in B16 melanoma cells. Our data showed that NFAT2 expression was increased in the hypopigmented B16 cells, while tyrosinase and MITF expression was decreased. To investigate the potential role of NFAT2, the recombinant adenovirus expressing microRNA specific for NFAT2 was transduced into the cultured B16 melanoma cells. Consistently, inhibition of NFAT2 enhanced tyrosinase activity and melanin content. Moreover, cyclosporine A, which is known as a calcineurin inhibitor blocking NFAT activation, enhanced tyrosinase activity and melanin content. These data suggest that NFAT2 may play an important role in regulation of melanogenesis in melanocyte.

Keyword

Melanogenesis; Melanocyte; B16 melanoma cells; NFAT2; Cyclosprorin A

MeSH Terms

Adenoviridae
Blotting, Western
Calcineurin
Cyclosporine
Down-Regulation
European Continental Ancestry Group
Hair
Humans
Hydroquinones
Melanins
Melanocytes
Melanoma, Experimental
Microarray Analysis
MicroRNAs
Monophenol Monooxygenase
NFATC Transcription Factors
Proteins
T-Lymphocytes
Transcription Factors
Transcriptional Activation
Calcineurin
Cyclosporine
Hydroquinones
Melanins
MicroRNAs
Monophenol Monooxygenase
NFATC Transcription Factors
Proteins
Transcription Factors

Figure

  • Fig. 1 Expression of NFAT2, tyrosinase, and MITF proteins in the hypopigmented B16 melanoma cells. (A) Photographs of the pellets from the B16 melanoma cells. Cells (2×106) were seeded onto 100mm dishes and cultured for 2 to 3 days [passage (P)1]. When the cells reached approximately 90% confluence, they were trypsinized, counted, and passaged into new dishes (P2). These procedures were repeated 2 times over a period of 2 weeks (P3, P5). Each culture was prepared for following experiment. (B) Expression of NFAT2, tyrosinase, and MITF in the hypopigmented B16 cells. Cell lysates from each culture were separated on polyacrylamide gels, transferred onto nitrocellulose membranes, and then reacted with the anti-NFAT2, anti-tyrosinase and anti-MITF antibodies. Anti-actin antibody was used as a loading control.

  • Fig. 2 Knock-down of NFAT2 by infection of adenovirus expressing microRNA specific for NFAT2. (A) The B16 cells were transduced with adenovirus expressing microRNA specific for NFAT2 (Ad/miNFAT2) at the 10 multiplicity of infection (MOI) for 6 h. After washing twice with PBS, cells were refed with growth medium and incubated for 24 h. The expression of NFAT2 was verified by Western blot using the anti-NFAT2 antibody and anti-actin antibody. Adenovirus expressing scrambled microRNA (Ad/miScr) was used as a negative control. (B) Cell survival was measured by WTS assay. Data are expressed as percentage of control (Ad/miScr at 1 day). The mean values±SEM are averages of triplicate measurements. *Statistically significant difference (P<0.05).

  • Fig. 3 Effect of NFAT2 knockdown on melanogenesis in B16 melanoma cells. The B16 cells were transduced with adenovirus expressing scrambled microRNA (Ad/miScr) and microRNA specific for NFAT2 (Ad/miNFAT2) at the 10 multiplicity of infection (MOI) for 6 h. After incubation for 48 h, alteration of pigmentation by NFAT2 knockdown verified by melanin content assay (A) and tyrosinase activity (B). Data are expressed as percentage of control (Ad/miScr). The mean values±SEM are averages of triplicate measurements. *Statistically significant difference (P<0.05).

  • Fig. 4 Effect of CsA on melanogenesis in B16 melanoma cells. The B16 cells were transduced with adenovirus expressing scrambled microRNA (Ad/miScr) and microRNA specific for NFAT2 (Ad/miNFAT2) at the 10 multiplicity of infection (MOI) for 6 h. After incubation for 24 h, Cells were further incubated with 20 µM of CsA. After 24h, melanin content assay (A) and tyrosinase activity (B) were performed as described in the materials and methods. The control was assessed by measurement of protein concentration. Data are expressed as percentage of control. The mean values±SEM are averages of triplicate measurements. *Statistically significant difference (P<0.05).


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