Lab Anim Res.  2011 Dec;27(4):365-368. 10.5625/lar.2011.27.4.365.

Antioxidative and aldose reductase-inhibitory effects of a fermentation filtrate of Rubus coreanus

Affiliations
  • 1Food Safety Support Organization, Korea Food Industry Association, Seoul, Korea.
  • 2College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea. solar93@cbu.ac.kr

Abstract

Antioxidative and aldose reductase (AR)-inhibitory effects of a fermentation filtrate of Rubus coreanus (FRC) were investigated using corneal/retinal homogenate and lens cytosol, respectively. Rat corneal/retinal homogenate was treated with 50 microM FeCl3 in the presence of FRC (3.2-100 microg/mL) for 30 min at 37degrees C, and thiobarbituric acid-reactive substances (TBARS) was quantified as a lipid peroxidation parameter. FRC markedly suppressed the TBARS production in a concentration-dependent manner, leading to 50% (IC50) and 100% (IC100) inhibitory concentrations of 20 and 95 microg/mL, respectively, which was similar to the effect of butylated hydroxyanisole. Activity of AR from rat lens was assayed in the presence of FRC (1-31.6 microg/mL) at 25degrees C using glyceraldehyde as a substrate. FRC inhibited lens AR by 50% (IC50) and 90% (IC90) at approximately 2 and 31.6 microg/mL, respectively, comparable to the effect of quercetin. The results indicate that ERC could be a promising candidate for the improvement of eye injury and visual dysfunction of dry eye and diabetic patients.

Keyword

Rubus coreanus; antioxidative activity; aldose reductase inhibition
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