Int J Oral Biol.  2012 Sep;37(3):109-114.

Identification of differentially expressed genes using an annealing control primer system in periodontitis

Affiliations
  • 1Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan, Korea. jchung@pusan.ac.kr

Abstract

In the gingival tissues of patients with periodontitis, inflammatory responses are mediated by a wide variety of genes. In this study, we screened for differentially expressed genes (DEGs) in periodontitis compared with normal tissue using an annealing control primer (ACP) system. By ACP RT-PCR analysis, we obtained about 160 amplicons, 8 of which were found to be differentially expressed. DEGs in patients with periodontitis were thus successfully and reliably identified by the ACP-based RT PCR technique. The DEGs identified in the screen may also enhance our understanding of the pathogenesis of periodontitis.

Keyword

periodontitis ACP-PCR; differentially expressed genes
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