J Vet Sci.  2012 Sep;13(3):253-259. 10.4142/jvs.2012.13.3.253.

Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times

• Zhang M
• Yue Z
• Liu Z
• Islam A
• Rehana B
• Tang S
• Bao E
• Hartung J

• KMID: 1389764
• DOI: http://doi.org/10.4142/jvs.2012.13.3.253

Abstract

The aim of this study was to assess changes of Hsp70 and HSF-1 protein and mRNA expression in stress-sensitive organs of pigs during transportation for various periods of time. Twenty pigs were randomly divided into four groups (0 h, 1 h, 2 h, and 4 h of transportation). A significant increased activity of AST and CK was observed after 1 h and 2 h of transportation. Histopathological changes in the heart, liver, and stomach indicated that these organs sustained different degrees of injury. Hsp70 protein expression in the heart and liver of transported pigs did not change significantly while it increased significantly (p < 0.05) in the stomach. Hsp70 mRNA levels decreased significantly (p < 0.05) in the heart after 4 h of transportation. However, mRNA expression increased significantly in the liver after 1 (p < 0.05) and 4 h (p < 0.01) of transportation, and increased significantly in the stomach of the transported pigs after 1, 4 (p < 0.01), and 2 h (p < 0.05). HSF-1 levels were reduced at 1 and 4 h (p < 0.05) only in the hearts of transported pigs. These results indicate that Hsp70 mediates distinct stress-related functions in different tissues during transportation.

Keyword

heat shock factor-1; heat shock protein 70; pig transport; stress-sensitive organs; transport time

MeSH Terms

Animals
Creatine Kinase/blood
DNA-Binding Proteins/*metabolism
Enzyme-Linked Immunosorbent Assay/veterinary
HSP70 Heat-Shock Proteins/*metabolism
Liver/*metabolism
Myocardium/*metabolism
RNA, Messenger/metabolism
Random Allocation
Real-Time Polymerase Chain Reaction/veterinary
Stomach/*metabolism
Stress, Physiological
Swine/blood/*metabolism
Time Factors
Transaminases/blood
Transcription Factors/*metabolism
*Transportation

Figure

• 

  Fig. 1 Representative images of hepatocytes from control and transported pigs. (A) Hepatocytes of a non-transported (control) pig. (B) Obvious granular and vacuolar degeneration characterized by increased cell size, faint and lightly stained cytoplasm, and expanded intracellular spaces (arrow) was observed in the cytoplasm of hepatocytes from pigs transported for 1 h. (C) Enlarged granular and vacuolar degeneration (arrow) in the cytoplasm of hepatocytes from pigs transported for 2 h. (D) Obvious necrotic hepatocytes characterized by karyopyknosis and karyolysis (arrow) in the hepatic lobule of pigs transported for 4 h. H&E stain. Scale bars = 10 µm.

• 

  Fig. 2 Representative images of myocardial cells from control and transported pigs. (A) Myocardial fibers in a non-transported (control) pig. (B) Granular degeneration (arrow) in the cytoplasm of myocardial fibers from a pig after 1 h of transportation. (C) Granular degeneration of myocardial cells indicated by light pink stpaining, tiny granular particles, and loss of striations in the cytoplasm (arrow) after 2 h of transportation. (D) Acute exudation characteristic of granular degeneration (arrow) among myocardial cells from pigs after 4 h of transportation. H&E stain. Scale bars = 10 µm.

• 

  Fig. 3 HSF-1 protein levels in the heart, liver, and stomach of transported pigs. *p < 0.05 and **p < 0.01 compared to the 0 h. Values are presented as the mean ± SD.

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