Korean J Parasitol.
2002 Mar;40(1):59-64. 10.3347/kjp.2002.40.1.59.
Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs
- Affiliations
-
- 1Veterinary and Agrochemical Research Centre, Brussels, Belgium.
- 2Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University, Mainz, Germany.
- KMID: 1384713
- DOI: http://doi.org/10.3347/kjp.2002.40.1.59
Abstract
- A Cryptosporidium parvum sporozoite and oocyst lambda gt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicity of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cryptosporidium immune calves and not by sera from parasite naive animals.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Concurrent response to challenge infection with Cryptosporidium parvum in immunosuppressed C57BL/6N mice
- Comparison of Resistance to gamma-Irradiation between Cryptosporidium parvum and Cryptosporidium muris Using In Vivo Infection
- PIAS1 interacts with the KRAB zinc finger protein, ZNF133, via zinc finger motifs and regulates its transcriptional activity
- Time gap between oocyst shedding and antibody responses in mice infected with Cryptosporidium parvum
- Diagnosis of bovine cryptosporidiosis by indirect immunofluorescence assay using monoclonal antibody
