Korean J Parasitol.  2002 Mar;40(1):59-64. 10.3347/kjp.2002.40.1.59.

Specific bovine antibody response against a new recombinant Cryptosporidium parvum antigen containing 4 zinc-finger motifs

Affiliations
  • 1Veterinary and Agrochemical Research Centre, Brussels, Belgium.
  • 2Institute of Medical Microbiology and Hygiene, Johannes Gutenberg-University, Mainz, Germany.

Abstract

A Cryptosporidium parvum sporozoite and oocyst lambda gt11 cDNA library was screened with a hyperimmune rabbit serum that was developed against insoluble fragments of ultrasonicated oocysts. A clone named Cp22.4.1 encoding a protein of 231 amino acids with 4 zinc-finger domains characterized by a Cys-X2-Cys-X4-His-X4-Cys motif was isolated and characterized. There was a complete match between the sequencing data of the coding region of Cp22.4.1 and the corresponding gene at chromosomal level. Cloning in a pBAD-TOPO-TA expression vector permitted to evaluate the antigenicity of the recombinant His-tagged antigen. This antigen was recognized by 2 out of 5 sera from Cryptosporidium immune calves and not by sera from parasite naive animals.

Keyword

Cryptosporidium; Antigen; Gene cloning; Zinc-finger; Bovine
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