J Vet Sci.  2006 Mar;7(1):47-51. 10.4142/jvs.2006.7.1.47.

Concurrent response to challenge infection with Cryptosporidium parvum in immunosuppressed C57BL/6N mice

• Surl CG
• Kim HC

• KMID: 1103553
• DOI: http://doi.org/10.4142/jvs.2006.7.1.47

Abstract

We investigated the response to challenge infection with Cryptosporidium parvum oocysts in immunosuppressed C57BL/6N mice. In the primary infection, fecal oocyst shedding and parasite colonization were greater in immunosuppressed mice than in nonimmunosuppressed mice. Compared with primary infection, challenge infection with C. parvum didn't show any oocyst shedding and parasite colonization. Especially, oocyst shedding and parasite colonization from the mice infected with heatkilled oocysts were not detected. After challenge infection with C. parvum oocysts, however, these mice were shedding small numbers of oocysts and parasite colonization. Except normal control and uninfected groups, the antibody titers of other groups appear similar. Based on the fecal oocyst shedding, parasite colonization of ilea, and antibody titers in the mice, these results suggest that the resistance to challenge infection with C. parvum in immunosuppressed C57BL/6N mice has increased.

Keyword

antibody response; Cryptosporidium parvum; immunosuppression; infection

MeSH Terms

Animals
Antibodies, Protozoan/blood
Cryptosporidiosis/*immunology/*parasitology
Cryptosporidium parvum/*immunology
Dexamethasone/immunology
Enzyme-Linked Immunosorbent Assay
Feces/parasitology
Female
Fluorescent Antibody Technique, Indirect
Histocytochemistry
Ileum/parasitology
Immunocompromised Host
Mice
Mice, Inbred C57BL
Oocysts/immunology
Random Allocation