J Korean Med Sci.  2000 Jun;15(3):273-278. 10.3346/jkms.2000.15.3.273.

Activated platelets induce secretion of interleukin-1beta, monocyte chemotactic protein-1, and macrophage inflammatory protein-1alpha and surface expression of intercellular adhesion molecule-1 on cultured endothelial cells

• Cha JK
• Jeong MH
• Bae HR
• Han JY
• Jeong SJ
• Jin HJ
• Lim YJ
• Kim SH
• Kim JW

• KMID: 1128623
• DOI: http://doi.org/10.3346/jkms.2000.15.3.273

Abstract

Atherosclerosis is an inflammatory disease. Platelet-endothelium interaction plays an important role in the pathophysiology of atherogenesis. We investigated the role of activated platelets for secretion of interleukin (IL)-1beta, monocyte chemotactic protein (MCP)-1 and macrophage inflammatory protein (MIP)-1alpha and expression of intercellular adhesion molecule (ICAM)-1 on endothelial cells. Human umbilical vein endothelial cells (HUVEC) were incubated with non-stimulated or ADP-activated platelets for 6 hr. Secretion of interleukin (IL)-1beta, MCP-1 and MIP-1alpha and surface expression of ICAM-1 were measured by ELISA and flow cytometry. In the presence of activated platelets, the secretion of IL-1beta, MCP-1, and MIP-1alpha and surface expression of ICAM-1 were significantly increased compared with non-activated platelets. The present study shows that activated platelets may contribute to expression of various inflammatory mediators on endothelial cells.

Keyword

Blood Platelets; Endothelium; Cell Adhesion Molecules; Atherosclerosis

MeSH Terms

Blood Platelets/metabolism*
Cells, Cultured
Cells, Cultured
Endothelium, Vascular/secretion*
Endothelium, Vascular/cytology
Human
Intercellular Adhesion Molecule-1/biosynthesis
Interleukin-1/secretion*
Macrophage Inflammatory Protein-1/secretion*
Monocyte Chemoattractant Protein-1/secretion*
Platelet Activation/physiology*