Exp Mol Med.  2006 Oct;38(5):509-518.

Inhibitory effect of Hsp70 on angiotensin II-induced vascular smooth muscle cell hypertrophy

• Zheng Y
• Im CN
• Seo JS

Abstract

Angiotensin II (Ang II), which is an important mediator of both vascular responsiveness and growth, has been shown to induce vascular smooth muscle cell (VSMC) hypertrophy via the activation of a complex series of intracellular signaling events. Heat shock protein 70 (Hsp70) has recently been shown to protect against Ang II-induced hypertension. In this study, we tested the hypothesis that Hsp70 can protect VSMC from Ang II-induced hypertrophy. We treated VSMCs with Ang II to induce hypertrophy and to activate MAPK signaling pathway. We observed that the augmentation of Hsp70 expression inhibited Ang II-stimulated VSMC hypertrophy. This inhibitory effect of Hsp70 appears to be partly due to extracellular signal-regulated kinase (ERK1/2) inactivation, which in turn, may possibly result from the accumulation of MAP kinase phosphatase-1 (MKP-1).

Keyword

angiotensin II; heat shock protein 70; hypertrophy; mitogen-activated protein kinases; muscle, smooth, vascular

MeSH Terms

Rats, Sprague-Dawley
Rats
RNA, Small Interfering/pharmacology
Protein-Tyrosine-Phosphatase/metabolism/physiology
Phosphoprotein Phosphatase/metabolism/physiology
Muscle, Smooth, Vascular/*cytology/*drug effects
Mitogen-Activated Protein Kinase 3/antagonists & inhibitors
Mitogen-Activated Protein Kinase 1/antagonists & inhibitors
Male
MAP Kinase Kinase 2/metabolism
MAP Kinase Kinase 1/metabolism
Immediate-Early Proteins/metabolism/physiology
Hypertrophy
HSP70 Heat-Shock Proteins/antagonists & inhibitors/*pharmacology
Flavonoids/pharmacology
Enzyme Stability/drug effects
Cells, Cultured
Cell Cycle Proteins/metabolism/physiology
Aorta/drug effects/pathology
Animals
Angiotensin II/*pharmacology